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Publication
µQuEChERS Combined with UHPLC-PDA as a State-of-the-Art Analytical Approach for Quantification of Chlorpropham in Potato
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Advisor(s)
Abstract(s)
Pesticides are chemicals used in agriculture to prevent insects, fungi, weeds, and other
pests, from damaging crops. In addition, some types of pesticides are used after harvest as sprout
suppressant agents help keeping the quality parameters of crops during storage. Nonetheless,
its presence, even at trace levels, in food products is becoming a big challenge regarding human
health. The current work aimed to develop and validate a sensitive and high-throughput analytical
approach, based on a state-of-the-art microextraction technique—µQuEChERS, combined with
ultra-high performance liquid chromatography equipped with a photodiode array detection system
(UHPLC-PDA) to quantify isopropyl-N-(3-chlorophenyl) carbamate (chlorpropham), commonly used
as efficient sprout suppressant stored potatoes, in raw and cooked potatoes cultivated in different
geographical regions of Madeira Island (Portugal). Good results were obtained in terms of figures of
the merit of the method, with correlation coefficients (R2
) higher than 0.999 and recoveries between
94.5% to 125%. Method limit of detection (LOD) and limit of quantification (LOQ) were 0.14 µg/Kg
and 0.43 µg/Kg, respectively, which are much lower than the accepted and legislated requirements
by the European Union, which is 20 µg/Kg for chlorpropham. The concentration of chlorpropham
in raw potatoes is significantly higher when compared to cooked samples, which revealed that the
thermic treatment during cooking had a significant effect on its degradation. A significant decrease
(90%, on average) was observed on chlorpropham levels.
Description
Keywords
Herbicide Chlorpropham Potato µQuEChERS/UHPLC-PDA Validation Cooking . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira
Citation
Gomes, T.M.; Perestrelo, R.; Câmara, J.S. µQuEChERS Combined with UHPLC-PDA as a State-of-the Art Analytical Approach for Quantification of Chlorpropham in Potato. Separations 2022, 9, 77. https://doi.org/10.3390/ separations9030077
Publisher
MDPI