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Optimisation of solid-phase microextraction combined with gas chromatography–mass spectrometry based methodology to establish the global volatile signature in pulp and skin of Vitis vinifera L. grape varieties

2011-09Journal article Open access
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dc.contributor.authorPerestrelo, Rosa Maria de Sá
dc.contributor.authorBarros, António S.
dc.contributor.authorRocha, Sílvia M.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2015-12-15T15:22:22Z
dc.date.available2015-12-15T15:22:22Z
dc.date.issued2011-09
dc.description.abstractThe volatiles (VOCs) and semi-volatile organic compounds (SVOCs) responsible for aroma are mainly present in skin of grape varieties. Thus, the present investigation is directed towards the optimisation of a solvent free methodology based on headspace-solid-phase microextraction (HS-SPME) combined with gas chromatography–quadrupole mass spectrometry (GC–qMS) in order to establish the global volatile composition in pulp and skin of Bual and Bastardo Vitis vinifera L. varieties. A deep study on the extraction-influencing parameters was performed, and the best results, expressed as GC peak area, number of identified compounds and reproducibility, were obtained using 4 g of sample homogenised in 5 mL of ultra-pure Milli-Q water in a 20 mL glass vial with addition of 2 g of sodium chloride (NaCl). A divinylbenzene/carboxen/polydimethylsiloxane fibre was selected for extraction at 60 °C for 45 min under continuous stirring at 800 rpm. More than 100 VOCs and SVOCs, including 27 monoterpenoids, 27 sesquiterpenoids, 21 carbonyl compounds, 17 alcohols (from which 2 aromatics), 10 C13 norisoprenoids and 5 acids were identified. The results showed that, for both grape varieties, the levels and number of volatiles in skin were considerably higher than those observed in pulp. According to the data obtained by principal component analysis (PCA), the establishment of the global volatile signature of grape and the relationship between different part of grapes—pulp and skin, may be an useful tool to winemaker decision to define the vinification procedures that improves the organoleptic characteristics of the corresponding wines and consequently contributed to an economic valorization and consumer acceptance.pt_PT
dc.identifier.citationPerestrelo, R., Barros, A. S., Rocha, S. M., & Câmara, J. S. (2011). Optimisation of solid-phase microextraction combined with gas chromatography–mass spectrometry based methodology to establish the global volatile signature in pulp and skin of Vitis vinifera L. grape varieties. Talanta, 85(3), 1483-1493.pt_PT
dc.identifier.doi10.1016/j.talanta.2011.06.025pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/961
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAVALIAÇÃO DO POTENCIAL DE AROMA DE CASTAS VITIS VINIFERA L. USADAS NA ELABORAÇÃO DE VINHOS TERRAS MADEIRENSES. INFLUÊNCIA DA ADIÇÃO DE ENZIMAS EXÓGENOS E DA FRACÇÃO POLIMÉRICA NAS PROPRIEDADES DE AROMA DOS VINHOS
dc.subjectVitis vinifera L. grapespt_PT
dc.subjectPulppt_PT
dc.subjectSkinpt_PT
dc.subjectHS-SPME/GC–qMSpt_PT
dc.subjectPCApt_PT
dc.subjectVolatile signaturept_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleOptimisation of solid-phase microextraction combined with gas chromatography–mass spectrometry based methodology to establish the global volatile signature in pulp and skin of Vitis vinifera L. grape varietiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAVALIAÇÃO DO POTENCIAL DE AROMA DE CASTAS VITIS VINIFERA L. USADAS NA ELABORAÇÃO DE VINHOS TERRAS MADEIRENSES. INFLUÊNCIA DA ADIÇÃO DE ENZIMAS EXÓGENOS E DA FRACÇÃO POLIMÉRICA NAS PROPRIEDADES DE AROMA DOS VINHOS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F38855%2F2007/PT
oaire.citation.endPage1493pt_PT
oaire.citation.startPage1483pt_PT
oaire.citation.titleTalantapt_PT
oaire.citation.volume85(3)pt_PT
person.familyNamePerestrelo
person.familyNameBarros
person.familyNameRocha
person.familyNameCâmara
person.givenNameRosa
person.givenNameAntónio
person.givenNameSílvia M.
person.givenNameJosé
person.identifier1441319
person.identifierR-000-200
person.identifierG-3003-2013
person.identifier.ciencia-id251A-D5F7-9E32
person.identifier.ciencia-id4714-1135-72CC
person.identifier.ciencia-id7B1E-AE7B-1100
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-7223-1022
person.identifier.orcid0000-0002-9103-5852
person.identifier.orcid0000-0002-0396-3019
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridI-2307-2014
person.identifier.scopus-author-id16686828800
person.identifier.scopus-author-id56130256800
person.identifier.scopus-author-id7006653213
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication003ff241-47d2-497b-b9ac-c0d1713f8bf3
relation.isAuthorOfPublication8a8ece98-d504-4c6b-8723-f77e82582d38
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