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RGD peptide-modified dendrimer-entrapped gold nanoparticles enable highly efficient and specific gene delivery to stem cells

2015-03-04Journal article Restricted access
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dc.contributor.authorKong, Lingdan
dc.contributor.authorAlves, Carla S.
dc.contributor.authorHou, Wenxiu
dc.contributor.authorQiu, Jieru
dc.contributor.authorMöhwald, Helmuth
dc.contributor.authorTomás, Helena
dc.contributor.authorShi, Xiangyang
dc.date.accessioned2019-07-10T10:35:35Z
dc.date.available2019-07-10T10:35:35Z
dc.date.issued2015-03-04
dc.description.abstractWe report the use of arginine-glycine-aspartic (Arg-Gly-Asp, RGD) peptide-modified dendrimer-entrapped gold nanoparticles (Au DENPs) for highly efficient and specific gene delivery to stem cells. In this study, generation 5 poly(amidoamine) dendrimers modified with RGD via a poly(ethylene glycol) (PEG) spacer and with PEG monomethyl ether were used as templates to entrap gold nanoparticles (AuNPs). The native and the RGD-modified PEGylated dendrimers and the respective well characterized Au DENPs were used as vectors to transfect human mesenchymal stem cells (hMSCs) with plasmid DNA (pDNA) carrying both the enhanced green fluorescent protein and the luciferase (pEGFPLuc) reporter genes, as well as pDNA encoding the human bone morphogenetic protein-2 (hBMP-2) gene. We show that all vectors are capable of transfecting the hMSCs with both pDNAs. Gene transfection using pEGFPLuc was demonstrated by quantitative Luc activity assay and qualitative evaluation by fluorescence microscopy. For the transfection with hBMP-2, the gene delivery efficiency was evaluated by monitoring the hBMP-2 concentration and the level of osteogenic differentiation of the hMSCs via alkaline phosphatase activity, osteocalcin secretion, calcium deposition, and von Kossa staining assays. Our results reveal that the stem cell gene delivery efficiency is largely dependent on the composition and the surface functionality of the dendrimer-based vectors. The coexistence of RGD and AuNPs rendered the designed dendrimeric vector with specific stem cell binding ability likely via binding of integrin receptor on the cell surface and improved three-dimensional conformation of dendrimers, which is beneficial for highly efficient and specific stem cell gene delivery applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationKong, L., Alves, C. S., Hou, W., Qiu, J., Möhwald, H., Tomás, H., & Shi, X. (2015). RGD peptide-modified dendrimer-entrapped gold nanoparticles enable highly efficient and specific gene delivery to stem cells. ACS applied materials & interfaces, 7(8), 4833-4843.pt_PT
dc.identifier.doi10.1021/am508760wpt_PT
dc.identifier.issn1944-8244
dc.identifier.urihttp://hdl.handle.net/10400.13/2473
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationDENDIMAGE - Development of Novel Dendrimer-Based Nanoparticles for Dual Mode Computed Tomography and Magnetic Resonance Imaging of Tumors
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectDendrimerspt_PT
dc.subjectGold nanoparticlespt_PT
dc.subjectGene deliverypt_PT
dc.subjectStem cellspt_PT
dc.subjectOsteogenic differentiationpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleRGD peptide-modified dendrimer-entrapped gold nanoparticles enable highly efficient and specific gene delivery to stem cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDENDIMAGE - Development of Novel Dendrimer-Based Nanoparticles for Dual Mode Computed Tomography and Magnetic Resonance Imaging of Tumors
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-NAN%2F1748%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.citation.endPage4843pt_PT
oaire.citation.startPage4833pt_PT
oaire.citation.titleACS Applied Materials and Interfacespt_PT
oaire.citation.volume7(8)pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameKong
person.familyNameAlves
person.familyNameTomás
person.familyNameShi
person.givenNameLingdan
person.givenNameCarla Sophia
person.givenNameHelena
person.givenNameXiangyang
person.identifier556975
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.ciencia-idBA11-1437-B948
person.identifier.orcid0000-0003-4209-8408
person.identifier.orcid0000-0002-8891-5234
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0001-6785-6645
person.identifier.ridE-5991-2010
person.identifier.ridA-1289-2007
person.identifier.scopus-author-id56539181900
person.identifier.scopus-author-id11438813200
person.identifier.scopus-author-id6508104177
person.identifier.scopus-author-id7402953116
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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