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Simultaneous analysis of free amino acids and biogenic amines in honey and wine samples using in loop orthophthalaldeyde derivatization procedure

2008-05Artigo científico Acesso aberto
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dc.contributor.authorPereira, Vanda Nulita Gomes
dc.contributor.authorPontes, Marisela
dc.contributor.authorCâmara, J. S.
dc.contributor.authorMarques, J. C.
dc.date.accessioned2015-12-15T14:19:12Z
dc.date.available2015-12-15T14:19:12Z
dc.date.issued2008-05
dc.description.abstractThis work presents a RP-HPLC method for the simultaneous quantification of free amino acids and biogenic amines in liquid food matrices and the results of the application to honey and wine samples obtained from different production processes and geographic origins. The developed methodology is based on a pre-column derivatization with o-phthaldialdehyde carried out in the sample injection loop. The compounds were separated in a Nova-Pack RP-C18 column (150 mm × 3.9 mm, 4 μm) at 35 °C. The mobile phase used was a mixture of phase A: 10 mM sodium phosphate buffer (pH 7.3), methanol and tetrahydrofuran (91:8:1); and phase B: methanol and phosphate buffer (80:20), with a flow rate of 1.0 ml/min. Fluorescence detection was used at an excitation wavelength of 335 nm and an emission wavelength of 440 nm. The separation and quantification of 19 amino acids and 6 amines was carried out in a single run as their OPA/MCE derivatives elute within 80 min, ensuring a reproducible quantification. The method showed to be adequate for the purpose, with an average RSD of 2% for the different amino acids; detection limits varying between 0.71 mg/l (Asn) and 8.26 mg/l (Lys) and recovery rates between 63.0% (Cad) and 98.0% (Asp). The amino acids present at the highest concentration in honey and wine samples were phenylalanine and arginine, respectively. Only residual levels of biogenic amines were detected in the analysed samples.pt_PT
dc.identifier.citationPereira, V., Pontes, M., Câmara, J. S., & Marques, J. C. (2008). Simultaneous analysis of free amino acids and biogenic amines in honey and wine samples using in loop orthophthalaldeyde derivatization procedure. Journal of Chromatography A, 1189(1-2), 435-443.pt_PT
dc.identifier.doi10.1016/j.chroma.2007.12.014pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/959
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationO EFEITO DA ESTUFAGEM NA FORMAÇÃO DE COMPOSTOS AROMÁTICOS CARACTERÍSTICOS DO VINHO MADEIRA
dc.subjectAmino acidspt_PT
dc.subjectBiogenic aminespt_PT
dc.subjectHoneypt_PT
dc.subjectWinept_PT
dc.subjectOPApt_PT
dc.subjectRP-HPLCpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleSimultaneous analysis of free amino acids and biogenic amines in honey and wine samples using in loop orthophthalaldeyde derivatization procedurept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleO EFEITO DA ESTUFAGEM NA FORMAÇÃO DE COMPOSTOS AROMÁTICOS CARACTERÍSTICOS DO VINHO MADEIRA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI-2010/SFRH%2FBD%2F24177%2F2005/PT
oaire.citation.endPage443pt_PT
oaire.citation.startPage435pt_PT
oaire.citation.titleJournal of Chromatography Apt_PT
oaire.citation.volume1189(1-2)pt_PT
oaire.fundingStreamPOCI-2010
person.familyNamePereira
person.familyNameCâmara
person.familyNameMarques
person.givenNameVanda
person.givenNameJosé
person.givenNameJose C
person.identifier41146
person.identifierG-3003-2013
person.identifier.ciencia-id0115-071E-87E8
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.ciencia-id1713-6DA1-A551
person.identifier.orcid0000-0003-0772-8953
person.identifier.orcid0000-0003-1965-3151
person.identifier.orcid0000-0002-4832-4341
person.identifier.ridJ-8697-2013
person.identifier.ridF-3014-2010
person.identifier.scopus-author-id36857887700
person.identifier.scopus-author-id10140393000
person.identifier.scopus-author-id7203033313
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication08f4685f-b917-410a-b153-59911ff7d75e
relation.isAuthorOfPublication.latestForDiscoverye10d78be-e547-4d25-92b5-06a997ed78da
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