O-coumaric acid ester, a potential early signaling molecule in Pinus pinea and Pisolithus arhizus symbiosis established in vitro

Ragonezi, C.; Teixeira, D.; Caldeira, A. T.; Martins, M .R.; Santos-Silva, C.; Ganhão, E.; Klimaszewska, K.; Zavattieri, M. A.

http://hdl.handle.net/10400.13/4063

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
O coumaric acid ester a potential early signaling molecule in Pinus pinea and Pisolithus arhizus symbiosis established in vitro.pdf		782.79 KB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

During ectomycorrhizal (ECM) establishment, biochemical signals lead to the development of complex structures in both the plant and the fungus that ultimately result in the formation of an ectomycorrhiza. The cross-talk between partners begins before physical contact. Our objective was to investigate the chemical nature of the signals during the first stages of in vitro mycorrhization of Pinus pinea with Pisolithus arhizus. For this purpose a double-phase solid liquid medium was expressly developed for the co-culture in order to simplify the extraction and further molecules analysis. O-coumaric acid ester was identified using HPLC UV and LC DAD MS on the second day of co-culture and its presence was detected for up to 10 days. These results contribute to the characterization of biochemical signals during pre-colonization involving conifer species and an ECM fungus, and demonstrate the suitability of the double-phase medium developed for the growth of both organisms and for the analysis of released chemical mediators.

Keywords

Adventitious rooting Co-cultivation Ectomycorrhiza Phenolics Pre-colonization Stone pine . Escola Superior de Tecnologias e Gestão

URI

http://hdl.handle.net/10400.13/4063

Citation

Ragonezi, C., Teixeira, D., Caldeira, A. T., Martins, M. D. R., Santos-Silva, C., Ganhão, E., ... & Zavattieri, M. A. (2014). O-coumaric acid ester, a potential early signaling molecule in Pinus pinea and Pisolithus arhizus symbiosis established in vitro. Journal of Plant Interactions, 9(1), 297-305.