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Separation of poly(amidoamine) (PAMAM) dendrimer generations by dynamic coating capillary electrophoresis

2006Journal article Restricted access
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dc.contributor.authorSedláková, P.
dc.contributor.authorSvobodová, J.
dc.contributor.authorMikšík, I.
dc.contributor.authorTomás, H.
dc.date.accessioned2019-07-05T14:43:40Z
dc.date.available2019-07-05T14:43:40Z
dc.date.issued2006
dc.description.abstractThe separation of compounds possessing amino groups (peptides, proteins, polyamino compounds) by capillary zone electrophoresis suffers from the interaction (sticking) of these solutes with the capillary wall. This sticking can result in the absence or incomplete separation of compounds or even in their retention in the capillary. Polyamidoamine (PAMAM) dendrimers are a class of spherical polymers with primary amino groups at the surface. These compounds can be separated reasonably well at acidic pH but not at neutral pH. A new method based on the dynamic coating of the capillary was developed for the separation of these compounds at pH 7.4. The method comprises separation in a fused-silica capillary (57 cm total length, 50 cm to the detector, ID 75 microm) and a background electrolyte consisting of a Tris-phosphate buffer (50 mmol/L, pH 7.4) and 0.05% (w/v) polyethyleneimine. This system is suitable for the separation of 7 generations of dendrimers (generations 0-6). The dynamic coating agent (polyethyleneimine) also improves the separation at acid pH.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSedlakova, P., Svobodova, J., Mikšík, I., & Tomás, H. (2006). Separation of poly(amidoamine) (PAMAM) dendrimer generations by dynamic coating capillary electrophoresis. Journal of Chromatography B, 841(1-2), 135-139.pt_PT
dc.identifier.doi10.1016/j.jchromb.2006.03.013pt_PT
dc.identifier.issn1570-0232
dc.identifier.urihttp://hdl.handle.net/10400.13/2455
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectPAMAM dendrimerspt_PT
dc.subjectDynamic coatingpt_PT
dc.subjectCapillary wallpt_PT
dc.subjectCapillary electrophoresispt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleSeparation of poly(amidoamine) (PAMAM) dendrimer generations by dynamic coating capillary electrophoresispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage139pt_PT
oaire.citation.startPage135pt_PT
oaire.citation.titleJournal of Chromatography Bpt_PT
oaire.citation.volume841(1-2)pt_PT
person.familyNameTomás
person.givenNameHelena
person.identifier556975
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id6508104177
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3a747114-6ab9-454b-985b-00cbf792e273
relation.isAuthorOfPublication.latestForDiscovery3a747114-6ab9-454b-985b-00cbf792e273

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