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Calcium phosphate-mediated gene delivery using simulated body fluid (SBF)

2012Journal article Restricted access
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dc.contributor.authorNouri, Alireza
dc.contributor.authorCastro, Rita
dc.contributor.authorSantos, José L.
dc.contributor.authorFernandes, César
dc.contributor.authorRodrigues, João
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-06-19T10:47:09Z
dc.date.available2019-06-19T10:47:09Z
dc.date.issued2012
dc.description.abstractThe present study aimed at developing a new approach in gene delivery of calcium phosphate nanoparticles through simulated body fluid (CaP-SBF). The physicochemical and biological characteristics of the CaP-SBF nanoparticles were compared with those made in pure water (CaP-water) via a similar procedure. The CaP-SBF and CaP-water solutions were then adjusted to two different pH values of 7.4 and 8.0, mixed with plasmid DNA (pDNA), and added in varying amounts to human embryonic kidney (HEK 293T) cells. The transfection efficiency and cell viability were studied in vitro by reporter gene (luciferase and Enhanced Green Fluorescent Protein) expression and the resazurin reduction assay, respectively, 24 and 48 h after the incubation with the nanoparticles. Our results indicated considerably high in vitro transfection efficiency for CaP-SBF/DNA complexes at physiological pH (7.4) with high amounts of CaP. Additionally, the SBF solution exhibited the ability to reduce the rapid growth of CaP particles over time, leading to higher transfection efficiency of CaP-SBF/DNA complexes than those made in water (CaP-water/DNA).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNouri, A., Castro, R., Santos, J. L., Fernandes, C., Rodrigues, J., & Tomás, H. (2012). Calcium phosphate-mediated gene delivery using simulated body fluid (SBF). International journal of pharmaceutics, 434(1-2), 199-208.pt_PT
dc.identifier.doi10.1016/j.ijpharm.2012.05.066pt_PT
dc.identifier.issn0378-5173
dc.identifier.urihttp://hdl.handle.net/10400.13/2409
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.relationBIOMIMETIC PREPARATION OF CALCIUM PHOSPHATE NANOPARTICLES USING DENDRIMERS AS TEMPLATES - APPLICATION ON GENE DELIVERY
dc.subjectSimulated body fluids (SBF)pt_PT
dc.subjectCalcium phosphatespt_PT
dc.subjectGene deliverypt_PT
dc.subjectTransfectionpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleCalcium phosphate-mediated gene delivery using simulated body fluid (SBF)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardTitleBIOMIMETIC PREPARATION OF CALCIUM PHOSPHATE NANOPARTICLES USING DENDRIMERS AS TEMPLATES - APPLICATION ON GENE DELIVERY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F47369%2F2008/PT
oaire.citation.endPage208pt_PT
oaire.citation.startPage199pt_PT
oaire.citation.titleInternational Journal of Pharmaceuticspt_PT
oaire.citation.volume434pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameNouri
person.familyNameCastro
person.familyNameFernandes
person.familyNameRodrigues
person.familyNameTomás
person.givenNameAlireza
person.givenNameRita
person.givenNameCesar
person.givenNameJoão
person.givenNameHelena
person.identifier556975
person.identifier.ciencia-id8314-881E-4986
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0003-3532-1309
person.identifier.orcid0000-0002-9337-062X
person.identifier.orcid0000-0001-9289-9962
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridA-2497-2013
person.identifier.ridB-6816-2008
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id14064572200
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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