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A sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in wines

2012-08Journal article Open access
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dc.contributor.authorSilva, Catarina L.
dc.contributor.authorGonçalves, João L.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2015-12-09T16:31:08Z
dc.date.available2015-12-09T16:31:08Z
dc.date.issued2012-08
dc.description.abstractA new approach based on microextraction by packed sorbent (MEPS) and reversed-phase high-throughput ultra high pressure liquid chromatography (UHPLC) method that uses a gradient elution and diode array detection to quantitate three biologically active flavonols in wines, myricetin, quercetin, and kaempferol, is described. In addition to performing routine experiments to establish the validity of the assay to internationally accepted criteria (selectivity, linearity, sensitivity, precision, accuracy), experiments are included to assess the effect of the important experimental parameters such as the type of sorbent material (C2, C8, C18, SIL, and C8/SCX), number of extraction cycles (extract-discard), elution volume, sample volume, and ethanol content, on the MEPS performance. The optimal conditions of MEPS extraction were obtained using C8 sorbent and small sample volumes (250 μL) in five extraction cycle and in a short time period (about 5 min for the entire sample preparation step). Under optimized conditions, excellent linearity View the MathML source(Rvalues2>0.9963), limits of detection of 0.006 μg mL−1 (quercetin) to 0.013 μg mL−1 (myricetin) and precision within 0.5–3.1% were observed for the target flavonols. The average recoveries of myricetin, quercetin and kaempferol for real samples were 83.0–97.7% with relative standard deviation (RSD, %) lower than 1.6%. The results obtained showed that the most abundant flavonol in the analyzed samples was myricetin (5.8 ± 3.7 μg mL−1). Quercetin (0.97 ± 0.41 μg mL−1) and kaempferol (0.66 ± 0.24 μg mL−1) were found in a lower concentration. The optimized MEPSC8 method was compared with a reverse-phase solid-phase extraction (SPE) procedure using as sorbent a macroporous copolymer made from a balanced ratio of two monomers, the lipophilic divinylbenzene and the hydrophilic N-vinylpyrrolidone (Oasis HLB) were used as reference. MEPSC8 approach offers an attractive alternative for analysis of flavonols in wines, providing a number of advantages including highest extraction efficiency (from 85.9 ± 0.9% to 92.1 ± 0.5%) in the shortest extraction time with low solvent consumption, fast sample throughput, more environmentally friendly and easy to perform.pt_PT
dc.identifier.citationSilva, C. L., Gonçalves, J. L., & Câmara, J. S. (2012). A sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in wines. Analytica chimica acta, 739, 89-98.pt_PT
dc.identifier.doi10.1016/j.aca.2012.06.020pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/943
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectWinespt_PT
dc.subjectFlavonolspt_PT
dc.subjectMicroextraction by packed sorbentpt_PT
dc.subjectSolid phase extractionpt_PT
dc.subjectUltra high pressure liquid chromatographypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleA sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in winespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage98pt_PT
oaire.citation.startPage89pt_PT
oaire.citation.titleAnalytica Chimica Actapt_PT
oaire.citation.volume739pt_PT
person.familyNameSousa Luís
person.familyNameGonçalves
person.familyNameCâmara
person.givenNameCatarina Grace
person.givenNameJoão
person.givenNameJosé
person.identifierC-1300-2019
person.identifierG-3003-2013
person.identifier.ciencia-id9813-3B88-BA8D
person.identifier.ciencia-id8C14-3188-9E44
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-3018-3165
person.identifier.orcid0000-0002-1265-6686
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridC-1348-2019
person.identifier.scopus-author-id57194492726
person.identifier.scopus-author-id52163735200
person.identifier.scopus-author-id10140393000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2853e6b6-0fb5-454c-aedb-05528b76c484
relation.isAuthorOfPublication7971c475-3f08-4462-96b1-79e96c9615a4
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscovery2853e6b6-0fb5-454c-aedb-05528b76c484

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