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Publication
Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae)
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Advisor(s)
Abstract(s)
The leafhopper genus Alebra Fieber, 1872 comprises a complex of morphologically similar species. The chromosome
complements (karyotypes) of five Alebra species, i.e. A. albostriella, A. coryli, A. viridis, A. wahlbergi and a new, yet undescribed
species, provisionally named Taxon 1, were here investigated, three of these species (A. coryli, A. viridis, and Taxon 1) for the first
time. The techniques applied included standard chromosome staining, fluorescence in situ hybridization (FISH) for mapping of 18S
rDNA and telomeric repeats (in every species), C-banding, AgNOR-banding and CMA3
/DAPI- staining (in A. viridis). The species
have a holokinetic type of chromosomes, as in other hemipterans. Karyotypes of all species are remarkably conserved with 2n = 22 +
X(0)/XX (male/female), one large and 10 medium pairs of autosomes and the X chromosome similar in size to larger chromosomes
within this group. In every species, FISH identified the “classical” insect telomere repeat of TTAGG and rRNA gene clusters located
on the homologues of a medium-sized pair of autosomes, presumably number 5. Thus, speciation in Alebra has apparently not involved
significant karyotypic changes. In A. viridis, rDNA sites were both Ag- and CMA3
-positive and were located at an interstitial position.
C-banding revealed heterochromatic bands in the X chromosome and also in all but four pairs of autosomes, the bands were located at
one telomere of a chromosome. C-bands were positive for CMA3
and negative for DAPI, suggesting that C-heterochromatin is mainly
enriched in GC-pairs.
Description
Keywords
Hemiptera Cicadellidae Alebra Karyotype Holokinetic chromosomes C-heterochromatin CMA3 /DAPI Ag-NOR FISH 18S rDNA TTAGG . Faculdade de Ciências da Vida
Citation
Kuznetsova, V. G., Maryańska-Nadachowska, A. N. N. A., Anokhin, B., & Aguin-Pombo, D. (2015). Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae). European Journal of Entomology, 112(2). doi: 10.14411/eje.2015.045
Publisher
Institute of Entomology