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Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia

2006Journal article Open access
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dc.contributor.authorPopova, Tatiana
dc.contributor.authorCarvalho, Miguel Â. A. Pinheiro de
dc.contributor.authorMatasova, Larisa
dc.contributor.authorMedvedeva, Liliya
dc.date.accessioned2021-03-18T14:27:47Z
dc.date.available2021-03-18T14:27:47Z
dc.date.issued2006
dc.description.abstractThe changes in the regulation of at mitochondrial NADP-isocitrate dehydrogenase (NADP-ICDH) in a rat heart during have been analysed. Increase of enzyme activity in the cytosol and mitochondria of the heart ischemia was detected. Catalytic properties of the mitochondrial NADP-ICDH at norm and pathology have been compared on homogeneous enzyme preparations. Enzyme from the normoxic and ischemic heart showed the same electrophoretical mobility and molecular mass. Enzyme isolated from the ischemic heart mitochondria demonstrated higher activation energy and lower thermal stability. NADP-isocitrate dehydrogenase at the normoxic and ischemic conditions exhibited different Km for substrates and regulatory behaviour in relation to ATP, ADP, 2-oxoglutarate, citrate, malate, reduced and oxidised glutathione. The inhibitory effect of the Fe2+ and H2O2 mixture associated with the generation of hydroxyl radicals was lower in the ischemic enzyme. We hypothesise that the specific features of regulation behaviour of NADP-ICDH from the ischemic tissues permits the enzyme to supply NADPH to the glutathione reductase/glutathione peroxidase system.pt_PT
dc.description.sponsorshipThis work was supported by grants from the Scientific Programs of Russia Ministry Education and Science (number of projects are RNP. 2.1.1.4429 and UR. 07.01.004).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPopova, T., Carvalho, M. A. P., Matasova, L., & Medvedeva, L. (2007). Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia. Molecular and cellular biochemistry, 294(1), 97-105.DOI: 10.1007/s11010-006-9249-9pt_PT
dc.identifier.doi10.1007/s11010-006-9249-9pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3181
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectIschemiapt_PT
dc.subjectOxidative stresspt_PT
dc.subjectNADP-isocitrate dehydrogenasept_PT
dc.subjectEnzyme regulationpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleRegulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemiapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage105pt_PT
oaire.citation.issue1-2pt_PT
oaire.citation.startPage97pt_PT
oaire.citation.titleMolecular and Cellular Biochemistrypt_PT
oaire.citation.volume294pt_PT
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameMiguel Angelo
person.identifier1218558
person.identifier.ciencia-id4610-6741-6816
person.identifier.orcid0000-0002-5084-870X
person.identifier.ridAEE-0913-2022
person.identifier.scopus-author-id8758577600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e433c48-88f8-40a2-b21f-de78b44aab1c
relation.isAuthorOfPublication.latestForDiscovery2e433c48-88f8-40a2-b21f-de78b44aab1c

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