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Determination of lipophilic and hydrophilic antioxidants in Solanum Lycopersicum L. tomato from Gordal variety by LL-USAE combined with UHPLC-PDA/FLR

datacite.subject.fosCiências Naturais::Ciências Químicaspt_PT
dc.contributor.advisorCâmara, José de Sousa
dc.contributor.authorFigueira, José Aldónio Oliveira
dc.date.accessioned2016-02-01T17:00:15Z
dc.date.available2016-03-02T01:30:10Z
dc.date.issued2014-11
dc.description.abstractTomato (Lycopersicon esculentum L.) is one of the main constituents of the Mediterranean diet. Its consumption has been proposed to reduce the risk of cardiovascular diseases and certain types of cancer. It is therefore one of the most popular and extensively consumed vegetable crop worldwide. To gain insights on the potential of Lycopersicon esculentum L. as bioactive food, two analytical methodologies were developed to determine the levels of the lipophilic -tocopherol, α-tocopherol, β-carotene, lycopene; and hydrophilic antioxidants ascorbic acid. The quantification of total carotenoids (β-carotene and lycopene) was assessed through a liquid–liquid ultrasound assisted extraction (LL-USAE) in combination with ultraviolet-visible spectroscopy (UV-Vis), according to method of mean, for total carotenoids (λmáx = 450 nm. The ultra-high performance liquid chromatographic using both photodiode array and fluorescence detection (UHPLC-PDA/FLR), allows the identification and quantification of the target lipophilic and hydrophilic antioxidants. This methodology UHPLC-PDA/FLR is fast, simple and revealed a high sensitivity for the compounds under study. The limits of detection (LODs) and quantification (LOQs) obtained were much lower (about 10 times) than the reported in literature. The method LL-USAE/UV-Vis was validated and applied to different tomato foodstuffs. The results reveal a small increase of carotenoids content during maturation, reaching the maximum level when ripe. These results complement those obtained by the ORAC and TBARS assays that show an increase of antioxidant capacity during maturation. The LODs ans LOQs obtained were also about 10 times lower than reported in literature. The carotenoid content was also evaluated by LL-USAE/UV-Vis in different tomatoes varieties. Regional variety present the high carotenoid level, followed by campari and gordal, and at last grape. This methodology was also applied to different processed food samples containing tomatoes derivatives. Highest carotenoids content were obtained in concentrated tomato foodstuffs.pt_PT
dc.identifier.tid201127989
dc.identifier.urihttp://hdl.handle.net/10400.13/1042
dc.language.isoengpt_PT
dc.relationMadeira Chemistry Research Centre
dc.subjectLycopersicon esculentum L.pt_PT
dc.subjectLipophilic antioxidantspt_PT
dc.subjectHydrophilic antioxidantspt_PT
dc.subjectRipeningpt_PT
dc.subjectUHPLCpt_PT
dc.subjectLL-USAEpt_PT
dc.subjectMethod validationpt_PT
dc.subjectApplied Biochemistrypt_PT
dc.subject.pt_PT
dc.subjectCentro de Ciências Exatas e da Engenhariapt_PT
dc.titleDetermination of lipophilic and hydrophilic antioxidants in Solanum Lycopersicum L. tomato from Gordal variety by LL-USAE combined with UHPLC-PDA/FLRpt_PT
dc.typemaster thesis
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F00674%2F2013/PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameOliveira Figueira
person.givenNameJosé Aldónio
person.identifierA-8724-2017
person.identifier.ciencia-id0B1E-B3DD-95F5
person.identifier.orcid0000-0003-3719-9327
person.identifier.scopus-author-id57201813505
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typemasterThesispt_PT
relation.isAuthorOfPublicationfc7f1e5e-1f91-4dd7-993c-f0e2063cc8c8
relation.isAuthorOfPublication.latestForDiscoveryfc7f1e5e-1f91-4dd7-993c-f0e2063cc8c8
relation.isProjectOfPublicationfd9571e0-489f-4866-8f87-99dadd8a09d5
relation.isProjectOfPublication.latestForDiscoveryfd9571e0-489f-4866-8f87-99dadd8a09d5
thesis.degree.nameMaster in Applied Biochemistrypt_PT

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