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Phenolic profile, toxicity, enzyme inhibition, in silico studies, and antioxidant properties of Cakile maritima scop.(Brassicaceae) from southern Portugal

2020Journal article Open access
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dc.contributor.authorPlacines, Chloé
dc.contributor.authorCastañeda-Loaiza, Viana
dc.contributor.authorRodrigues, Maria João
dc.contributor.authorPereira, Catarina G.
dc.contributor.authorStefanucci, Azzurra
dc.contributor.authorMollica, Adriano
dc.contributor.authorZengin, Gokhan
dc.contributor.authorLlorent-Martínez, Eulogio J.
dc.contributor.authorCastilho, Paula C.
dc.contributor.authorCustódio, Luísa
dc.date.accessioned2021-03-24T14:51:04Z
dc.date.available2021-03-24T14:51:04Z
dc.date.issued2020
dc.description.abstractCakile maritima Scop. (sea rocket) is an edible halophyte plant with several ethnomedicinal uses. This work reports the chemical profile and bioactivities of food grade extracts from sea rocket organs. Toxicity was determined on mammalian cells, and phenolic profiling and the quantitation of the main metabolites were made by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Enzymatic inhibition was determined towards acetyl- and butyrylcholinesterase (AChE, BuChE), α-glucosidase, α-amylase, and tyrosinase. Docking studies were performed to tyrosinase, on the major metabolites, and samples were tested for antioxidant properties. Extracts were not toxic, were constituted mainly by flavonoids, and some compounds (roseoside and oleuropein) are here described for the first time in the species. The aerial organs' ethanol extract had relevant activity towards 2,2-diphenyl-1-picrylhydrazyl [DPPH, half maximal inhibitory concentration (IC50) = 0.59 mg/mL], and ferric-reducing activity power (FRAP, IC50 = 0.99 mg/mL). All samples were more active towards AChE than on BuChE. The ethanol fruits' extract inhibited α-glucosidase [2.19 mmol of equivalent of acarbose (ACAE)/g]. Samples were active against tyrosinase, especially the aerial organs' ethanol extracts [25.9 mg of equivalent of kojic acid (KAE)/g]. Quercetin and kaempferol glycosides fit well into the enzymatic pocket of tyrosinase. Our results suggest sea rocket as a candidate to be further explored as a source of bioactive products.pt_PT
dc.description.sponsorshipAuthor Contributions: Funding acquisition, L.C.; Investigation, C.P., V.C.-L., M.J.R., A.S., A.M., G.Z., E.J.L.-M. and P.C.C.; Methodology, L.C.; Supervision, L.C.; Writing—original draft, C.P., M.J.R., C.G.P., A.S., A.M., G.Z., E.J.L.-M., P.C.C. and L.C.; Writing—review and editing, G.Z., E.J.L.-M., P.C.C. and L.C. All authors have read and agreed to the published version of the manuscript. Funding: This work was sustained by the Foundation for Science and Technology (FCT) and the Portuguese National and Regional Budgets (CCMAR/Multi/04326/2019 project), UID/DTP/04138/2019 and GreenVet project (ALG-01-0145-FEDER-028876). Maria João Rodrigues was funded by the PhD grant SFRH/BD/116604/2016. Luísa Custódio was supported by the FCT Scientific Employment Stimulus (CEECIND/00425/2017). Acknowledgments: Technical and human support provided by “El Centro de Instrumentación Científico-Técnica” (CICT) of Universidad de Jaén (UJA, MINECO, Junta de Andalucía, FEDER) is gratefully acknowledged.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPlacines, C., Castañeda-Loaiza, V., Rodrigues, M. J., Pereira, C. G., Stefanucci, A., Mollica, A., ... & Castilho, P. C. (2020). Phenolic profile, toxicity, enzyme inhibition, in silico studies, and antioxidant properties of Cakile maritima scop.(Brassicaceae) from southern Portugal. Plants, 9(142). https://doi.org/10.3390/plants9020142pt_PT
dc.identifier.doi10.3390/plants9020142pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3196
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationCentre of Marine Sciences
dc.relationResearch Institute for Medicines
dc.relationUnravelling the biotechnological potential of halophyte species from the Algarve coast - HaloBiotech
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBioactive plant-derived productspt_PT
dc.subjectTyrosinase inhibitorspt_PT
dc.subjectSalt tolerant plantspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titlePhenolic profile, toxicity, enzyme inhibition, in silico studies, and antioxidant properties of Cakile maritima scop.(Brassicaceae) from southern Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleUnravelling the biotechnological potential of halophyte species from the Algarve coast - HaloBiotech
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F116604%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00425%2F2017%2FCP1391%2FCT0001/PT
oaire.citation.issue142pt_PT
oaire.citation.titlePlantspt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStreamCEEC IND 2017
person.familyNameCastilho
person.givenNamePaula C
person.identifierA-4412-2013
person.identifier.ciencia-id6D15-8C69-A2E0
person.identifier.orcid0000-0002-8303-4286
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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