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Homocysteine metabolism in children and adolescents: influence of age on plasma biomarkers and correspondent genotype interactions

2019Journal article Open access
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dc.contributor.authorAraújo, Helena Caldeira
dc.contributor.authorRamos, Ruben
dc.contributor.authorFlorindo, Cristina
dc.contributor.authorRivera, Isabel
dc.contributor.authorCastro, Rita
dc.contributor.authorAlmeida, Isabel Tavares de
dc.date.accessioned2022-07-19T13:07:57Z
dc.date.available2022-07-19T13:07:57Z
dc.date.issued2019
dc.description.abstractBackground: Imbalance of homocysteine (Hcy) metabolism links with several pathologies; nevertheless, it is poorly characterized in pediatric populations. This study investigated the impact of age on plasma concentrations of Hcy and relevant biomarkers along with correspondent genotype interactions. Methods: A healthy pediatric cohort aged 9 (n = 195) and 17 (n = 128) years old (yo) was studied. Immunoassays and GC-MS-SIM-mode quantified plasma levels of Hcy and biomarkers. PCR-RFLP or quantitative-PCR assays assessed common variations in related genes. Results: Age impacted on levels of Hcy and metabolic markers: older children presented with the lowest folates and total-cobalamin (tCbl), while with the highest Hcy concentrations, whereas methylmalonic acid (MMA) and holotranscobalamin (Holo-TC) levels remained similar in 9-yo and 17-yo children. The relationships between B-vitamins and metabolic markers were also dependent on age. Only in the older children, MMA correlated with tCbl and Holo-TC, and MMA levels were markedly higher in the 17-yo subjects presenting with the lowest quartiles of Holo-TC concentrations. Lastly, age also impacted on the correlations between genotype and biomarkers. In the 17-yo group, however not in the 9-yo children, tHcy differed between MTHFR 677 genotypes, with subjects who had the MTHFR 677TT genotype displaying the highest tHcy concentrations. Conclusions: Age impacts on the Hcy metabolism dynamics in a pediatric population.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAraújo, H. C., Ramos, R., Florindo, C., Rivera, I., Castro, R., & Almeida, I. T. (2019). Homocysteine metabolism in children and adolescents: influence of age on plasma biomarkers and correspondent genotype interactions. Nutrients, 11(3), 646.pt_PT
dc.identifier.doi10.3390/nu11030646pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4425
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMadeira Chemistry Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectVitamin B12pt_PT
dc.subjectMethylmalonic acidpt_PT
dc.subjectFolatept_PT
dc.subjectHomocysteinept_PT
dc.subjectMTHFRpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleHomocysteine metabolism in children and adolescents: influence of age on plasma biomarkers and correspondent genotype interactionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F00674%2F2013/PT
oaire.citation.issue3pt_PT
oaire.citation.startPage646pt_PT
oaire.citation.titleNutrientspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCaldeira Araújo
person.familyNameCastro
person.givenNameHelena
person.givenNameRita
person.identifier.ciencia-id5E1E-E1EA-1B40
person.identifier.ciencia-id8314-881E-4986
person.identifier.orcid0000-0003-2212-4859
person.identifier.orcid0000-0002-9337-062X
person.identifier.ridA-2497-2013
person.identifier.scopus-author-id14063100400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationceb211d0-5522-42ba-bbf0-adfc23f37f84
relation.isAuthorOfPublication.latestForDiscovery0e0cca92-5886-4679-a79c-e358ea928b6c
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