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Profiling of volatiles in the leaves of Lamiaceae species based on headspace solid phase microextraction and mass spectrometry

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dc.contributor.authorSilva, Catarina L.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-18T10:13:37Z
dc.date.available2019-07-18T10:13:37Z
dc.date.issued2013
dc.description.abstractTo establish the volatile metabolomic profile of fresh leaves from Mentha × piperita L. and Mentha spicata L. aromatic plants, a headspace solid-phase microextraction (HS-SPME) technique combined with gas chromatography–mass spectrometry (GC–qMS) is reported. To assure the best extraction conditions, the influence of experimental parameters on the extraction efficiency, including the nature of fibre coatings, extraction temperature and extraction time, were investigated and optimized. The conditions that promoted the higher extraction efficiency, corresponding to the higher GC peak areas and number of identified metabolites were: DVB/CAR/PDMS coating fibre, 40 °C and 60 min as the extraction temperature and extraction time, respectively. Kovàts indices, mass spectra and/or standard compounds were used to identify up to 52 and 37 volatile organic metabolites (VOMs) in the headspace of M. × piperita L. and M. spicata L. fresh leaves, respectively, representing 91.26% and 88.52% of the total peak area of each plant. These VOMs belonging to different chemical families namely monoterpenes hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes, pyrazines, higher alcohols, ethyl esters and carbonyl compounds. D-Carvone (54.77%) was the most abundant component isolated from the headspace of M. × piperita L. fresh leaves, followed by D-limonene (13.10%) and (+)-epi-bicyclosesquiphellandrene (5.14%), whereas M. spicata L. leaves contained 2,5-dimethyl-3-methoxypyrazine (54.58%), caryophyllene (5.50%) and D-germacrene (4.75%), as the main volatile metabolites. The results suggested that the HS-SPME/GC–qMS methodology could be used to establish the similarities and differences between both investigated Lamiaceae plants, and could probably be used for plant differentiation based on the volatile metabolomic profile.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, C. L., & Câmara, J. S. (2013). Profiling of volatiles in the leaves of Lamiaceae species based on headspace solid phase microextraction and mass spectrometry. Food research international, 51(1), 378-387.pt_PT
dc.identifier.doi10.1016/j.foodres.2012.12.040pt_PT
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10400.13/2479
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectMentha × piperita L.pt_PT
dc.subjectMentha spicata Lpt_PT
dc.subjectVolatilespt_PT
dc.subjectMetabolomic signaturept_PT
dc.subjectSolid-phase microextractionpt_PT
dc.subjectGC–qMSpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleProfiling of volatiles in the leaves of Lamiaceae species based on headspace solid phase microextraction and mass spectrometrypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage387pt_PT
oaire.citation.startPage378pt_PT
oaire.citation.titleFood Research Internationalpt_PT
oaire.citation.volume51(1)pt_PT
person.familyNameSousa Luís
person.familyNameCâmara
person.givenNameCatarina Grace
person.givenNameJosé
person.identifierC-1300-2019
person.identifierG-3003-2013
person.identifier.ciencia-id9813-3B88-BA8D
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-3018-3165
person.identifier.orcid0000-0003-1965-3151
person.identifier.scopus-author-id57194492726
person.identifier.scopus-author-id10140393000
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2853e6b6-0fb5-454c-aedb-05528b76c484
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscovery2853e6b6-0fb5-454c-aedb-05528b76c484

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