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Optimization and validation of a micro–QuEChERS method for phthalates detection in small samples of cetacean blubber

2024Journal article Open access
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dc.contributor.authorSambolino, Annalisa
dc.contributor.authorRodriguez, Marta
dc.contributor.authorDe la Fuente, Jesus
dc.contributor.authorArbelo, Manuel
dc.contributor.authorFernández, Antonio
dc.contributor.authorKaufmann, Manfred
dc.contributor.authorCordeiro, Nereida
dc.contributor.authorDinis, Ana
dc.date.accessioned2024-02-27T15:27:26Z
dc.date.available2024-02-27T15:27:26Z
dc.date.issued2024
dc.description.abstractIn this study, an innovative method was developed to detect and quantify phthalates in fresh cetacean blubber. An adaptation of the ammonium formate QuEChERS method was used and adapted as a micro-extraction for small quantities of samples. Significantly, this technique utilized minimal quantities of reagents and salts, with the additional implementation of rigorous Quality Assurance/Quality Control protocols to further reduce background contamination. To ensure the reliability of this method, comprehensive validation procedures were conducted, with a specific focus on two widely studied cetacean species: the common bottlenose dolphin (Tursiops truncatus) and the short-finned pilot whale (Globicephala macrorhynchus). Determination coefficients (R2) for matrix-matched calibration were >0.93 with limits of quantifications (LOQ) of the method in the range of 5–10 ng/g. Mean recovery values were between 40 and 100 %. This novel methodology holds particular relevance for environmental research studies, offering the capability to detect emerging contaminants with minimal sample requirements. This aspect is particularly valuable in investigations that involve free-ranging animals and rely on biopsy sampling. It allows for the assessment of contaminant levels in healthy individuals within wild populations, enhancing our understanding of ecological impacts and potential conservation measures. • A micro-extraction adaptation of the ammonium formate QuEChERS method was developed and applied to a small quantity of fresh cetacean blubber to detect phthalates. • Small quantities of reagents and salts were used, and additional Quality Assurance/ Quality Control procedures were taken to further minimize background contamination. • Method validation was carried out for two cosmopolitan and extensively studied cetacean species: the common bottlenose dolphin (Tursiops truncatus) and the short-finned pilot whale (Globicephala macrorhynchus).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSambolino, A., Rodriguez, M., De la Fuente, J., Arbelo, M., Fernández, A., Kaufmann, M., ... & Dinis, A. (2024). Optimization and validation of a micro–QuEChERS method for phthalates detection in small samples of cetacean blubber. MethodsX, 12, 102502.pt_PT
dc.identifier.doi10.1016/j.mex.2023.102502pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/5586
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationMarine and Environmental Sciences Centre
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBottlenose dolphinpt_PT
dc.subjectPilot whalept_PT
dc.subjectPhthalic acid esterspt_PT
dc.subjectPlastic additivespt_PT
dc.subjectGC–MSpt_PT
dc.subjectGas chromatography-mass spectrometrypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleOptimization and validation of a micro–QuEChERS method for phthalates detection in small samples of cetacean blubberpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04423%2F2020/PT
oaire.citation.startPage102502pt_PT
oaire.citation.titleMethodsXpt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSambolino
person.familyNameKaufmann
person.familyNamecordeiro
person.familyNameDinis
person.givenNameAnnalisa
person.givenNameManfred
person.givenNamenereida
person.givenNameAna
person.identifier1612636
person.identifier.ciencia-idAC13-70D4-E99A
person.identifier.ciencia-idA316-A34A-F7AF
person.identifier.ciencia-id521A-0F62-EA39
person.identifier.ciencia-id0412-30C5-4376
person.identifier.orcid0000-0003-0231-1446
person.identifier.orcid0000-0001-6213-3229
person.identifier.orcid0000-0001-6006-3415
person.identifier.orcid0000-0002-1508-9197
person.identifier.ridA-7561-2013
person.identifier.ridO-2253-2019
person.identifier.scopus-author-id35264346100
person.identifier.scopus-author-id7004319456
person.identifier.scopus-author-id26665393900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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