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Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia

2012Journal article Open access
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dc.contributor.authorGouveia, Sandra C.
dc.contributor.authorCastilho, Paula C.
dc.date.accessioned2021-03-31T13:19:45Z
dc.date.available2021-03-31T13:19:45Z
dc.date.issued2012
dc.description.abstractThe caffeoylquinic acids (CQA) found in Helichrysum species from Madeira Archipelago were identified and quantified. The HPLC-DAD–ESI/MSn method applied was developed and validated showing a good repeatability with recovery values ≥96%. The use of a RP-C18 with a small internal diameter (Ø 3.0 mm) and an acidic mobile phase (acetonitrile and water with 0.1% (v/v) of formic acid) enabled the separation of all compounds within a 30 min analysis. A good resolution between 1,5-O-diCQA and 3,5-O-diCQA isomers, usually hard to separate, was also accomplished. Dicaffeoylquinic acids isomers were the major components among the quantified hydroxycinnamic acids. 1,5-O-diCQA, 3,5-O-diCQA and 5-O-CQA were the compounds found in higher amounts for the different species. The distinct uses of these plants described in the local folk medicine can be related to the phenolic composition.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGouveia, S. C., & Castilho, P. C. (2012). Validation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesia. Food research international, 45(1), 362-368.pt_PT
dc.identifier.doi10.1016/j.foodres.2011.09.023pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3233
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCanadian Institute of Food Science and Technology (CIFST)pt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.relationISOLAMENTO E CARACTERIZAÇÃO DE PRINCÍPIOS ACTIVOS DE PLANTAS PARA O COMBATE À TUBERCULOSE
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCaffeoylquinic acidspt_PT
dc.subjectHPLC-DAD–ESI/MSpt_PT
dc.subjectHelichrysumpt_PT
dc.subjectValidationpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleValidation of a HPLC-DAD–ESI/MSn method for caffeoylquinic acids separation, quantification and identification in medicinal Helichrysum species from Macaronesiapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardTitleISOLAMENTO E CARACTERIZAÇÃO DE PRINCÍPIOS ACTIVOS DE PLANTAS PARA O COMBATE À TUBERCULOSE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F24227%2F2005/PT
oaire.citation.endPage368pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage362pt_PT
oaire.citation.titleFood Research Internationalpt_PT
oaire.citation.volume45pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGouveia-Figueira
person.familyNameCastilho
person.givenNameSandra
person.givenNamePaula C
person.identifierA-4412-2013
person.identifier.ciencia-id6D15-8C69-A2E0
person.identifier.orcid0000-0002-8116-2794
person.identifier.orcid0000-0002-8303-4286
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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