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Comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry combined with solid phase microextraction as a powerful tool for quantification of ethyl carbamate in fortified wines. The case study of Madeira wine

2010-05Journal article Open access
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dc.contributor.authorPerestrelo, Rosa Maria de Sá
dc.contributor.authorPetronilho, Silvia
dc.contributor.authorCâmara, José S.
dc.contributor.authorRocha, Sílvia M.
dc.date.accessioned2015-12-07T15:11:08Z
dc.date.available2015-12-07T15:11:08Z
dc.date.issued2010-05
dc.description.abstractAn analytical methodology based on headspace solid phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography—time-of-flight mass spectrometry (GC × GC–ToFMS) was developed for the identification and quantification of the toxic contaminant ethyl carbamate (EC) directly in fortified wines. The method performance was assessed for dry/medium dry and sweet/medium sweet model wines, and for quantification purposes, calibration plots were performed for both matrices using the ion extraction chromatography (IEC) mode (m/z 62). Good linearity was obtained with a regression coefficient (r2) higher than 0.981. A good precision was attained (R.S.D. <20%) and low detection limits (LOD) were achieved for dry (4.31 μg/L) and sweet (2.75 μg/L) model wines. The quantification limits (LOQ) and recovery for dry wines were 14.38 μg/L and 88.6%, whereas for sweet wines were 9.16 μg/L and 99.4%, respectively. The higher performance was attainted with sweet model wine, as increasing of glucose content improves the volatile compound in headspace, and a better linearity, recovery and precision were achieved. The analytical methodology was applied to analyse 20 fortified Madeira wines including different types of wine (dry, medium dry, sweet, and medium sweet) obtained from several harvests in Madeira Island (Portugal). The EC levels ranged from 54.1 μg/L (medium dry) to 162.5 μg/L (medium sweet).pt_PT
dc.identifier.citationPerestrelo, R., Petronilho, S., Câmara, J. S., & Rocha, S. M. (2010). Comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry combined with solid phase microextraction as a powerful tool for quantification of ethyl carbamate in fortified wines. The case study of Madeira wine. Journal of Chromatography A, 1217(20), 3441-3445.pt_PT
dc.identifier.doi10.1016/j.chroma.2010.03.027pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/936
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAVALIAÇÃO DO POTENCIAL DE AROMA DE CASTAS VITIS VINIFERA L. USADAS NA ELABORAÇÃO DE VINHOS TERRAS MADEIRENSES. INFLUÊNCIA DA ADIÇÃO DE ENZIMAS EXÓGENOS E DA FRACÇÃO POLIMÉRICA NAS PROPRIEDADES DE AROMA DOS VINHOS
dc.subjectEthyl carbamatept_PT
dc.subjectFortified winept_PT
dc.subjectMadeira winept_PT
dc.subjectHS-SPMEpt_PT
dc.subjectGC × GC−ToFMSpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleComprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry combined with solid phase microextraction as a powerful tool for quantification of ethyl carbamate in fortified wines. The case study of Madeira winept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAVALIAÇÃO DO POTENCIAL DE AROMA DE CASTAS VITIS VINIFERA L. USADAS NA ELABORAÇÃO DE VINHOS TERRAS MADEIRENSES. INFLUÊNCIA DA ADIÇÃO DE ENZIMAS EXÓGENOS E DA FRACÇÃO POLIMÉRICA NAS PROPRIEDADES DE AROMA DOS VINHOS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F38855%2F2007/PT
oaire.citation.endPage3445pt_PT
oaire.citation.startPage3441pt_PT
oaire.citation.titleJournal of Chromatography Apt_PT
oaire.citation.volume1217(20)pt_PT
person.familyNamePerestrelo
person.familyNameCâmara
person.familyNameRocha
person.givenNameRosa
person.givenNameJosé
person.givenNameSílvia M.
person.identifier1441319
person.identifierG-3003-2013
person.identifier.ciencia-id251A-D5F7-9E32
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.ciencia-id7B1E-AE7B-1100
person.identifier.orcid0000-0002-7223-1022
person.identifier.orcid0000-0003-1965-3151
person.identifier.orcid0000-0002-0396-3019
person.identifier.ridI-2307-2014
person.identifier.scopus-author-id16686828800
person.identifier.scopus-author-id10140393000
person.identifier.scopus-author-id7006653213
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication003ff241-47d2-497b-b9ac-c0d1713f8bf3
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublicationb1862483-5d94-4f61-a7b8-0e640022d7df
relation.isAuthorOfPublication.latestForDiscoverye10d78be-e547-4d25-92b5-06a997ed78da
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