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Octadecyl functionalized core–shell magnetic silica nanoparticle as a powerful nanocomposite sorbent to extract urinary volatile organic metabolites

2015Journal article Restricted access
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dc.contributor.authorQiao, Zheng
dc.contributor.authorPerestrelo, Rosa
dc.contributor.authorReyes-Gallardo, Emilia M.
dc.contributor.authorLucena, R.
dc.contributor.authorCárdenas, S.
dc.contributor.authorRodrigues, João
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-19T14:03:49Z
dc.date.available2019-07-19T14:03:49Z
dc.date.issued2015
dc.description.abstractIn this present study, magnetic Fe3O4@SiO2 nanoparticles (MNPs) functionalized with octadecyl groups (Fe3O4@SiO2-C18 NPs) were synthesized, characterized and employed, for the first time, as powerful nanosorbent to extract endogenous volatile organic metabolites (EVOMs) namely, hexanal, heptanal, decanal, benzaldehyde, 4-heptanone, 5-methyl-2-furfural and phenol, described as potential biomarkers of cancer, from human urine. By using co-precipitation, surface modification methods, the carbon-ferromagnetic nanocomposite was synthesized and characterized by infrared spectrum (IR) and transmission electron microscopy (TEM). By coupling with gas chromatography-mass spectrometry (GC-qMS), a reliable, sensitive and cost-effective method was validated. To test the extraction efficiency of the carbon-ferromagnetic nanocomposite toward urinary EVOMs experimental variables affecting the extraction performance, including nanosorbent amount, adsorption time, elution time, and nature of elution solvent, were investigated in detail. The extraction process was performed by dispersing Fe3O4@SiO2-C18 NPs into working solution containing targeted VOMs, and into urine samples, and then eluted with an adequate organic solvent. The eluate was collected, concentrated and analyzed by GC-qMS. Under the optimized conditions, the LODs and LOQs achieved were in the range of 9.7-57.3 and 32.4-190.9ng/mL, respectively. Calibration curves were linear (r(2)≥0. 988) over the concentration ranges from 0.25 to 250ng/mL. In addition, a satisfying reproducibility was achieved by evaluating the intra- and inter-day precisions with relative standard deviations (RSDs) less than 3 and 11%, respectively. The method also afforded satisfactory results in terms of the matrix effect (72.8-96.1%) and recoveries (accuracy) higher than 75.1% for most of the studied EVOMs. The Fe3O4@SiO2-C18 NPs-based sorbent extraction combined with GC-qMS revealed that the new nanosorbent had a strong ability to retain the target metabolites providing a new, reliable and high throughput strategy for isolation of targeted EVOMs in human urine, suggesting their potential to be applied in other EVOMs.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationQiao, Z., Perestrelo, R., Reyes-Gallardo, E. M., Lucena, R., Cárdenas, S., Rodrigues, J., & Câmara, J. S. (2015). Octadecyl functionalized core–shell magnetic silica nanoparticle as a powerful nanocomposite sorbent to extract urinary volatile organic metabolites. Journal of Chromatography A, 1393, 18-25.pt_PT
dc.identifier.doi10.1016/j.chroma.2015.03.026pt_PT
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/10400.13/2490
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectEndogenous volatile organic metabolites (EVOMs)pt_PT
dc.subjectMagnetic nanoparticlespt_PT
dc.subjectMagnetic solid phase extractionpt_PT
dc.subjectUrine samplespt_PT
dc.subjectCancer biomarkerspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleOctadecyl functionalized core–shell magnetic silica nanoparticle as a powerful nanocomposite sorbent to extract urinary volatile organic metabolitespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/New-INDIGO%2F0003%2F2012/PT
oaire.citation.endPage25pt_PT
oaire.citation.startPage18pt_PT
oaire.citation.titleJournal of Chromatography Apt_PT
oaire.citation.volume1393pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNamePerestrelo
person.familyNameReyes Gallardo
person.familyNameRodrigues
person.familyNameCâmara
person.givenNameRosa
person.givenNameEmilia M.
person.givenNameJoão
person.givenNameJosé
person.identifier1441319
person.identifierG-3003-2013
person.identifier.ciencia-id251A-D5F7-9E32
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-7223-1022
person.identifier.orcid0000-0001-5563-8069
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridI-2307-2014
person.identifier.ridB-6816-2008
person.identifier.scopus-author-id16686828800
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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