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Expression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in rats

2013Journal article Open access
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dc.contributor.authorIskusnykh, Igor Y.
dc.contributor.authorPopova, Tatyana N.
dc.contributor.authorAgarkov, Aleksander A.
dc.contributor.authorCarvalho, Miguel Â. A. Pinheiro de
dc.contributor.authorRjevskiy, Stanislav G.
dc.date.accessioned2021-03-12T14:26:59Z
dc.date.available2021-03-12T14:26:59Z
dc.date.issued2013
dc.description.abstractCorrelation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4 administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationIskusnykh, I. Y., Popova, T. N., Agarkov, A. A., Carvalho, M. Â.P., & Rjevskiy, S. G. (2013). Expression of glutathione peroxidase and glutathione reductase and level of free radical processes under toxic hepatitis in rats. Journal of Toxicology, 2013, 277-285. http://dx.doi.org/10.1155/2013/870628pt_PT
dc.identifier.doi10.1155/2013/870628pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3166
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherHindawi Publishing Corporationpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGlutathione peroxidasept_PT
dc.subjectGlutathione reductasept_PT
dc.subjectHepatitis in ratspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleExpression of Glutathione peroxidase and Glutathione reductase and level of free radical processes under toxic hepatitis in ratspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Toxicologypt_PT
oaire.citation.volume2013pt_PT
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameMiguel Angelo
person.identifier1218558
person.identifier.ciencia-id4610-6741-6816
person.identifier.orcid0000-0002-5084-870X
person.identifier.ridAEE-0913-2022
person.identifier.scopus-author-id8758577600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e433c48-88f8-40a2-b21f-de78b44aab1c
relation.isAuthorOfPublication.latestForDiscovery2e433c48-88f8-40a2-b21f-de78b44aab1c

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