Repository logo
 
Publication

New method for determination of (E)resveratrol in wine based on microextraction using packed sorbent and ultra-performance liquid chromatography

2011-06Journal article Open access
Simple item page
dc.contributor.authorGonçalves, João
dc.contributor.authorCâmara, José S.
dc.date.accessioned2015-12-09T11:20:09Z
dc.date.available2015-12-09T11:20:09Z
dc.date.issued2011-06
dc.description.abstractAn ultra-fast and improved analytical methodology based on microextraction by packed sorbent (MEPS) combined with ultra-performance LC (UPLC) was developed and validated for determination of (E)-resveratrol in wines. Important factors affecting the performance of MEPS such as the type of sorbent material (C2, C8, C18, SIL, and M1), number of extraction cycles, and sample volume were studied. The optimal conditions of MEPS extraction were obtained using C8 sorbent and small sample volumes (50–250mL) in one extraction cycle (extract–discard) and in a short time period (about 3 min for the entire sample preparation step). (E)-Resveratrol was eluted by 1 250mL of the mixture containing 95% methanol and 5% water, and the separation was carried out on a highstrength silica HSS T3 analytical column (100 mm 2.1 mm, 1.8mm particle size) using a binary mobile phase composed of aqueous 0.1% formic acid (eluent A) and methanol (eluent B) in the gradient elution mode (10 min of total analysis). The method was fully validated in terms of linearity, detection (LOD) and quantification (LOQ) limits, extraction yield, accuracy, and inter/intra-day precision, using a Madeira wine sample (ET) spiked with (E)-resveratrol at concentration levels ranging from 5 to 60mg/mL. Validation experiments revealed very good recovery rate of 9575.8% RSD, good linearity with r2 values 40.999 within the established concentration range, excellent repeatability (0.52%), and reproducibility (1.67%) values (expressed as RSD), thus demonstrating the robustness and accuracy of the MEPSC8/UPLC-photodiode array (PDA) method. The LOD of the method was 0.21mg/mL, whereas the LOQ was 0.68mg/mL. The validated methodology was applied to 30 commercial wines (24 red wines and six white wines) from different grape varieties, vintages, and regions. On the basis of the analytical validation, the MEPSC8/UPLC-PDA methodology shows to be an improved, sensitive, and ultra-fast approach for determination of (E)-resveratrol in wines with high resolving power within 6 min.pt_PT
dc.identifier.citationGonçalves, J., & Câmara, J. S. (2011). New method for determination of (E)‐resveratrol in wine based on microextraction using packed sorbent and ultra‐performance liquid chromatography. Journal of separation science, 34(18), 2376-2384.pt_PT
dc.identifier.doi10.1002/jssc.201100336pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/939
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley-VCHpt_PT
dc.subject(E)-Resveratrolpt_PT
dc.subjectMicroextraction by packed sorbentpt_PT
dc.subjectUltraperformance LCpt_PT
dc.subjectWinept_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleNew method for determination of (E)resveratrol in wine based on microextraction using packed sorbent and ultra-performance liquid chromatographypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2384pt_PT
oaire.citation.startPage2376pt_PT
oaire.citation.titleJournal of separation sciencept_PT
oaire.citation.volume34(18)pt_PT
person.familyNameGonçalves
person.familyNameCâmara
person.givenNameJoão
person.givenNameJosé
person.identifierG-3003-2013
person.identifier.ciencia-id8C14-3188-9E44
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-1265-6686
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridC-1348-2019
person.identifier.scopus-author-id52163735200
person.identifier.scopus-author-id10140393000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7971c475-3f08-4462-96b1-79e96c9615a4
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscovery7971c475-3f08-4462-96b1-79e96c9615a4

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
New method for determination of (E)resveratrol in wine based on microextraction.pdf
Size:
240.49 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artigos em revistas internacionais