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Tissue engineering scaffold material with enhanced cell adhesion and angiogenesis from soy protein isolate loaded with bio modulated micro-TiO2 prepared via prolonged sonication for wound healing applications

2022Journal article Open access
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dc.contributor.authorKoshy, Rekha Rose
dc.contributor.authorMary, Siji K.
dc.contributor.authorReghunadhan, Arunima
dc.contributor.authorDalvi, Yogesh Bharat
dc.contributor.authorKailas, Lekshmi
dc.contributor.authorCordeiro, Nereida
dc.contributor.authorThomas, Sabu
dc.contributor.authorPothen, Laly A.
dc.date.accessioned2023-04-21T13:31:51Z
dc.date.available2023-04-21T13:31:51Z
dc.date.issued2022
dc.description.abstractTissue engineering is a technique that promotes healing by creating an ideal environment for endogenous cells to migrate and grow into the site of injury via a scaffold, improving regeneration and reducing the time required for in vitro cell culture. In this work, the effect of the addition of sonicated TiO2 in the soy protein isolate (SPI) matrix for tissue engineering applications was studied. In comparison to adding expensive nano TiO2, this method of incorporating sonicated TiO2 into the SPI matrix will aid in achieving improved properties at a lower cost. The effect of the addition of sonicated TiO2 on the morphological, UV transmittance, mechanical, thermal, surface energy, and hydrophilicity of SPI films was investigated. The result shows that the uniformly distributed TiO2 particles successfully blocked 95% of UV light. Scanning electron microscopy revealed a significant reduction in the TiO2 agglomerate size and homogeneous distribution of the same when sonication was applied instead of mechanical dispersion. A simultaneous increase of tensile strength (from 3.16 to 4.58 MPa) and elongation at break values (from 24.25% to 95.31%) with 0.5% TiO2 was observed. The addition of 0.25% TiO2 was found to significantly enhance the elongation at break value to 120.83%. Incorporation of micro-TiO2 particles could improve the surface roughness, surface energy, and wettability of SPI films. In vitro cell adhesion studies and in vivo subcutaneous implantation studies were performed to assess the cell growth and angiogenesis of the developed film membranes. An MTT assay showed that SPI-1%TiO2 film favored cell viability up to 118%, and in vivo subcutaneous implantation studies showed enhanced cell growth and angiogenesis for SPI-1% TiO2 films. This SPI-TiO2 film with enhanced surface properties can be used as an ideal candidate for tissue engineering applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1021/acsbiomaterials.2c00548pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/5133
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectSPIpt_PT
dc.subjectTiO2pt_PT
dc.subjectCell adhesionpt_PT
dc.subjectSonicationpt_PT
dc.subjectTissue engineeringpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleTissue engineering scaffold material with enhanced cell adhesion and angiogenesis from soy protein isolate loaded with bio modulated micro-TiO2 prepared via prolonged sonication for wound healing applicationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage4908pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage4896pt_PT
oaire.citation.titleACS Biomaterials Science & Engineeringpt_PT
oaire.citation.volume8pt_PT
person.familyNameReghunadhan
person.familyNamecordeiro
person.givenNameArunima
person.givenNamenereida
person.identifier1612636
person.identifier.ciencia-id521A-0F62-EA39
person.identifier.orcid0000-0003-3658-3589
person.identifier.orcid0000-0001-6006-3415
person.identifier.ridO-2253-2019
person.identifier.scopus-author-id7004319456
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication36dad165-d4fe-4c0c-a63b-841937d7fb30
relation.isAuthorOfPublication3d02b657-c48c-4f56-813a-1284b81960f3
relation.isAuthorOfPublication.latestForDiscovery36dad165-d4fe-4c0c-a63b-841937d7fb30

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