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Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors

2012Journal article Restricted access
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dc.contributor.authorShan, Yuebin
dc.contributor.authorLuo, Ting
dc.contributor.authorPeng, Chen
dc.contributor.authorSheng, Ruilong
dc.contributor.authorCao, Amin
dc.contributor.authorCao, Xueyan
dc.contributor.authorShen, Mingwu
dc.contributor.authorGuo, Rui
dc.contributor.authorTomás, Helena
dc.contributor.authorShi, Xiangyang
dc.date.accessioned2019-07-09T14:03:48Z
dc.date.available2019-07-09T14:03:48Z
dc.date.issued2012
dc.description.abstractDevelopment of highly efficient nonviral gene delivery vectors still remains a great challenge. In this study, we report a new gene delivery vector based on dendrimer-entrapped gold nanoparticles (Au DENPs) with significantly higher gene transfection efficiency than that of dendrimers without AuNPs entrapped. Amine-terminated generation 5 poly(amidoamine) (PAMAM) dendrimers (G5.NH(2)) were utilized as templates to synthesize AuNPs with different Au atom/dendrimer molar ratios (25:1, 50:1, 75:1, and 100:1, respectively). The formed Au DENPs were used to complex two different pDNAs encoding luciferase (Luc) and enhanced green fluorescent protein (EGFP), respectively for gene transfection studies. The Au DENPs/pDNA polyplexes with different N/P ratios and compositions of Au DENPs were characterized by gel retardation assay, light scattering, zeta potential measurements, and atomic force microscopic imaging. We show that the Au DENPs can effectively compact the pDNA, allowing for highly efficient gene transfection into the selected cell lines as demonstrated by both Luc assay and fluorescence microscopic imaging of the EGFP expression. The transfection efficiency of Au DENPs with Au atom/dendrimer molar ratio of 25:1 was at least 100 times higher than that of G5.NH(2) dendrimers without AuNPs entrapped at the N/P ratio of 2.5:1. The higher gene transfection efficiency of Au DENPs is primarily due to the fact that the entrapment of AuNPs helps preserve the 3-dimensional spherical morphology of dendrimers, allowing for more efficient interaction between dendrimers and DNA. With the less cytotoxicity than that of G5.NH(2) dendrimers demonstrated by thiazoyl blue tetrazolium bromide assay and higher gene transfection efficiency, it is expected that Au DENPs may be used as a new gene delivery vector for highly efficient transfection of different genes for various biomedical applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationShan, Y., Luo, T., Peng, C., Sheng, R., Cao, A., Cao, X., ... & Shi, X. (2012). Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors. Biomaterials, 33(10), 3025-3035.pt_PT
dc.identifier.doi10.1016/j.biomaterials.2011.12.045pt_PT
dc.identifier.issn0142-9612
dc.identifier.urihttp://hdl.handle.net/10400.13/2468
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectPAMAM dendrimerspt_PT
dc.subjectGold nanoparticlespt_PT
dc.subjectGene deliverypt_PT
dc.subjectTransfection efficiencypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleGene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectorspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3035pt_PT
oaire.citation.startPage3025pt_PT
oaire.citation.titleBiomaterialspt_PT
oaire.citation.volume33(10)pt_PT
person.familyNameSheng
person.familyNameCao
person.familyNameTomás
person.familyNameShi
person.givenNameRuilong
person.givenNameXueyan
person.givenNameHelena
person.givenNameXiangyang
person.identifierhttps://scholar.google.ca/citations?user=YCheaFsAAAAJ&hl=en
person.identifier556975
person.identifier.ciencia-idC019-C809-403B
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.ciencia-idBA11-1437-B948
person.identifier.orcid0000-0002-3303-3832
person.identifier.orcid0000-0003-4366-8943
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0001-6785-6645
person.identifier.ridE-5991-2010
person.identifier.ridA-1289-2007
person.identifier.scopus-author-id7005472737
person.identifier.scopus-author-id6508104177
person.identifier.scopus-author-id7402953116
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication447440d5-343e-4804-a4fb-98584bb0749c
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relation.isAuthorOfPublication3a747114-6ab9-454b-985b-00cbf792e273
relation.isAuthorOfPublicationc6b4b8c5-1241-4906-b4b9-ace5576b0b62
relation.isAuthorOfPublication.latestForDiscovery447440d5-343e-4804-a4fb-98584bb0749c

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