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Changes in fatty acid biosynthesis in marine microalgae as a response to medium nutrient availability
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Authors
Advisor(s)
Abstract(s)
To maximize and enhance the fatty acid (FA) profiles of microalgae, it is crucial to understand the metabolic path ways that lead to high levels of the desired product. Nannochloropsis gaditana, Rhodomonas marina, and Isochrysis
sp. were cultivated in media with various nutrient concentrations, and the biomass was analyzed for protein and
fatty acid contents. The long chain polyunsaturated fatty acid (LC-PUFA) and protein contents were maximized in
media with high nutrient concentrations. Under these conditions, the reduction in the (SFA + MUFA)/PUFA ratio
(by as much as 83%) indicated that R. marina and N. gaditana shifted preference towards PUFA synthesis. The
∑ω3/∑ω6 ratio indicated that R. marina preferentially synthesized ω3 FA towards high nutrient concentrations
(depicted by an eightfold increase) in contrast to Isochrysis sp. (which yielded a 35% reduction). In addition, the
limitations of LC-PUFA biosynthesis at the level of its precursors (e.g. C18:2ω6, C18:3ω3, C18:4ω3) and further
conversion to LC-PUFA as nutrients were increased, suggesting that external variations induced changes in the
sets of enzymes that maintain the desaturation and elongation pathways of FA. The present study provides
novel insights into the regulation of LC-PUFA biosynthesis and facilitates the modeling of microalgal FA patterns
depending on the field of application.
Description
Keywords
Nutrient availability LC-PUFA N. gaditana R. marina Isochrysis sp . Faculdade de Ciências Exatas e da Engenharia
Citation
Fernandes, T., Fernandes, I., Andrade, C. A., & Cordeiro, N. (2016). Changes in fatty acid biosynthesis in marine microalgae as a response to medium nutrient availability. Algal research, 18, 314-320.
Publisher
Elsevier