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Functionalization of poly(amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells

2010Journal article Restricted access
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dc.contributor.authorSantos, José L.
dc.contributor.authorOliveira, Hugo
dc.contributor.authorPandita, Deepti
dc.contributor.authorRodrigues, João
dc.contributor.authorPêgo, Ana P.
dc.contributor.authorGranja, Pedro L.
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-06-17T15:45:25Z
dc.date.available2019-06-17T15:45:25Z
dc.date.issued2010
dc.description.abstractA new family of gene delivery vectors is synthesized consisting of a medium-size generation PAMAM dendrimer (generation 5, with amine termini) core randomly linked at the periphery to hydrophobic chains that vary in length (12 to 16 carbon alkyl chains) and number (from 4.2 to 9.7 in average). The idea subjacent to the present work is to join the advantages of the cationic nature of the dendrimer with the capacity of lipids to interact with biological membranes. Unlike other amphiphilic systems designed for the same purpose, where the hydrophobic and hydrophilic moieties coexist in opposite sides, the present vectors have a hydrophilic interior and a hydrophobic corona. The vectors are characterized in respect to their ability to neutralize, bind and compact plasmid DNA (pDNA). The complexes formed between the vectors and pDNA are analyzed concerning their size, zeta-potential, resistance to serum nucleases, capacity of being internalized by cells and transfection efficiency. These new vectors show a remarkable capacity for mediating the internalization of pDNA with minimum cytotoxicity, being this effect positively correlated with the -CH(2)- content present in the hydrophobic corona. Gene expression in MSCs, a cell type with relevancy in the regenerative medicine clinical context, is also enhanced using the new vectors but, in this case, the higher efficiency is shown by the vectors containing the smallest hydrophobic chains.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSantos, J. L., Oliveira, H., Pandita, D., Rodrigues, J., Pêgo, A. P., Granja, P. L., & Tomás, H. (2010). Functionalization of poly (amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells. Journal of Controlled Release, 144, 55-64.pt_PT
dc.identifier.doi10.1016/j.jconrel.2010.01.034pt_PT
dc.identifier.issn0168-3659
dc.identifier.urihttp://hdl.handle.net/10400.13/2400
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationBONE TISSUE ENGINEERING VIA LOCAL GENE DELIVERY
dc.subjectPoly(amidoamine) dendrimerpt_PT
dc.subjectGene deliverypt_PT
dc.subjectMesenchymal stem cellspt_PT
dc.subjectCellular uptakept_PT
dc.subjectBiomedical applicationspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleFunctionalization of poly(amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBONE TISSUE ENGINEERING VIA LOCAL GENE DELIVERY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI-2010/SFRH%2FBD%2F19450%2F2004/PT
oaire.citation.endPage64pt_PT
oaire.citation.startPage55pt_PT
oaire.citation.titleJournal of Controlled Releasept_PT
oaire.citation.volume144pt_PT
oaire.fundingStreamPOCI-2010
person.familyNameRodrigues
person.familyNamePêgo
person.familyNameTomás
person.givenNameJoão
person.givenNameAna Paula
person.givenNameHelena
person.identifier164495
person.identifier556975
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id6114-92F0-E64C
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0001-5169-328X
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridB-6816-2008
person.identifier.ridC-6942-2012
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id56053889300
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication905a2f89-0018-47d3-850d-4b09bed65b5f
relation.isAuthorOfPublication3a747114-6ab9-454b-985b-00cbf792e273
relation.isAuthorOfPublication.latestForDiscovery3a747114-6ab9-454b-985b-00cbf792e273
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