Logo do repositório
 
Publicação

Nutrient deprivation stimulates soluble extracellular polymeric substances: physiological and biochemical responses in the cyanobacterium Cyanocohniella rudolphia

2026-04Artigo científico Acesso aberto
Ver registo simples
datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
dc.contributor.authorRodrigues, Filipa
dc.contributor.authorMendonça, Ivana
dc.contributor.authorFaria, Marisa
dc.contributor.authorMougin, Karine
dc.contributor.authorGómez Pinchetti, Juan Luis
dc.contributor.authorFerreira, Artur
dc.contributor.authorCordeiro, Nereida
dc.contributor.authorGonçalves Rodrigues, Ana Filipa
dc.contributor.authorSilva Mendonça, Ivana Rita da
dc.contributor.authorFaria, Marisa Camacho Gonçalves
dc.contributor.authorcordeiro, nereida
dc.date.accessioned2026-09-21T15:33:37Z
dc.date.available2026-09-21T15:33:37Z
dc.date.issued2026-04
dc.description.abstractThe effect of macro- and micronutrient deprivation on the cyanobacterium Cyanocohniella rudolphia (BEA 0786B) over 180 days (without medium renewal) was evaluated to intensify the production of soluble extracellular polymeric substances (S-EPS). Growth, pH, cell-free medium viscosity, S-EPS content (phenol‑sulfuric acid assay), Alcian Blue staining, and biochemical/structural markers were monitored to assess EPS release and extracellular matrix dynamics. Progressive nutrient depletion induced a shift from biomass growth toward extracellular-matrix investment. Microcolony formation observed around day 45 preceded a sustained high- production phase, with pronounced matrix densification evident by approximately day 75. Apparent viscosity increased in parallel with S-EPS accumulation and showed a strong correlation with extracellular carbohydrate concentration (R 2 = 0.86), supporting its use as an operational, non-destructive proxy for S-EPS. Alcian Blue staining qualitatively confirmed the presence of acidic polysaccharides. Quantitatively, S-EPS concentrations increased from 0.1 to 0.8 g L 1 , accompanied by an increase in apparent viscosity from 24.7 to 34.7 mPa⋅s. Morphological and spectroscopic analyses indicated a predominantly carbohydrate-rich extracellular matrix with an increasing protein contribution over time. As an exploratory proof of concept, unprocessed S-EPS present in the spent culture medium enabled LAP- initiated photochemical formation of Au and Ag nanoparticles at 365 nm, with S-EPS acting as a stabilising and co-reducing matrix and conferring pH-responsive optical behaviour. This indicates a practical route toward integration into EPS-rich matrices to engineer stimuli-responsive coatings and biomaterials. Overall, this study demonstrates that prolonged nutrient deprivation without medium renewal is an effective strategy to intensify S- EPS accumulation in C. rudolphia and that apparent viscosity provides a practical in-process parameter for monitoring production and supporting direct valorisation of spent culture medium in saline bioprocesses.eng
dc.identifier.citationRodrigues, F., Mendonça, I., Faria, M., Mougin, K., Pinchetti, J. L. G., Ferreira, A., & Cordeiro, N. (2026). Nutrient deprivation stimulates soluble extracellular polymeric substances: physiological and biochemical responses in the cyanobacteria Cyanocohniella rudolphia. Algal Research, 104600.
dc.identifier.doi10.1016/j.algal.2026.104600
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.13/7960
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relationDeveloping Bioactive and Intelligent Biomaterials from Microalgae/Cyanobacteria for Sustainable Food Packaging
dc.relationUID/PRR/04423/2025
dc.relationLA/P/0101/2020
dc.relation2024.01262.BD
dc.relation2023.04389.BD
dc.relation2020.06615.BD
dc.relation.ispartofAlgal Research
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCyanocohniella rudolphia
dc.subjectSoluble EPS (S-EPS)
dc.subjectNutrient deprivation
dc.subjectStarvation phase
dc.subjectViscosity proxy
dc.subjectSpent-medium valorisation
dc.subject.
dc.subjectFaculdade de Ciências Exatas e da Engenharia
dc.titleNutrient deprivation stimulates soluble extracellular polymeric substances: physiological and biochemical responses in the cyanobacterium Cyanocohniella rudolphiaeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumber2023.04389.BD
oaire.awardTitleDeveloping Bioactive and Intelligent Biomaterials from Microalgae/Cyanobacteria for Sustainable Food Packaging
oaire.awardURIhttp://hdl.handle.net/10400.13/7509
oaire.citation.titleAlgal Research
oaire.citation.volume95
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGonçalves Rodrigues
person.familyNameSilva Mendonça
person.familyNameFaria
person.familyNamecordeiro
person.givenNameAna Filipa
person.givenNameIvana Rita da
person.givenNameMarisa Camacho Gonçalves
person.givenNamenereida
person.identifier3435316
person.identifier1612636
person.identifier.ciencia-idA816-656A-6465
person.identifier.ciencia-id7619-C648-AE6C
person.identifier.ciencia-idD716-6F48-AD0E
person.identifier.ciencia-id521A-0F62-EA39
person.identifier.orcid0000-0001-7171-6012
person.identifier.orcid0000-0002-9148-026X
person.identifier.orcid0000-0002-9158-6961
person.identifier.orcid0000-0001-6006-3415
person.identifier.ridO-2253-2019
person.identifier.scopus-author-id7004319456
relation.isAuthorOfPublication4b8b827d-260f-401e-9062-b09ddcdb82fa
relation.isAuthorOfPublication832d1d80-719b-4935-b565-1d7c31c90f77
relation.isAuthorOfPublication57ef72f0-8e40-4853-b4ab-416884e127fa
relation.isAuthorOfPublication3d02b657-c48c-4f56-813a-1284b81960f3
relation.isAuthorOfPublication.latestForDiscovery4b8b827d-260f-401e-9062-b09ddcdb82fa
relation.isProjectOfPublication4563bd17-f203-46bf-931e-b2641f10e7ab
relation.isProjectOfPublication.latestForDiscovery4563bd17-f203-46bf-931e-b2641f10e7ab

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
Nutrient deprivation stimulates soluble extracellular polymeric substances.pdf
Tamanho:
3.39 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
4.03 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

Artigos em revistas internacionais