Repository logo
 
Publication

Molecular identification and VOMs characterization of Saccharomyces cerevisiae strains isolated from Madeira region winery environments

2020Journal article Open access
Simple item page
dc.contributor.authorCastillo, Mariangie
dc.contributor.authorSilva, Emanuel da
dc.contributor.authorCâmara, José S.
dc.contributor.authorKhadem, Mahnaz
dc.date.accessioned2022-10-06T09:10:29Z
dc.date.available2022-10-06T09:10:29Z
dc.date.issued2020
dc.description.abstractThe quality and typical characteristic of wines depends, among other factors, on the volatile organic metabolites (VOMs) that are biosynthesized by yeasts, mainly Saccharomyces cerevisiae species. The yeast strain influences the diversity and proportions of the VOMs produced during the fermentation process, as the genetic predisposition of the strains is a by-product of selective adaptation to the ecosystem. The present work reports the characterization of S. cerevisiae strains isolated from grape must, used in the Demarcated Region of Madeira (DRM) for winemaking. Yeast species were identified by amplification and by restriction fragment length polymorphism (RFLP) analysis of the region 5.8S-internal transcribed spacers (PCR-RFLP of 5.8S-ITS) of ribosomal DNA (rDNA). The strains identification was performed by analyzing the RFLP pattern of mitochondrial DNA (RFLP-mtDNA). The representative strains were selected for the characterization of the volatile profile through headspace solid-phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) analysis. A total of 77 VOMs were identified. Higher alcohols, esters, and fatty acids were the major chemical families representing 63%, 16%, and 9%, respectively, in strain A and 54%, 23%, and 15% in strain B. The results indicate the influence of the strain metabolism in the production of VOMs, many of which probably participate in the aroma of the corresponding wines.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationastillo, M., Silva, E. D., Câmara, J. S., & Khadem, M. (2020). Molecular identification and VOMs characterization of Saccharomyces cerevisiae strains isolated from Madeira region winery environments. Processes, 8(9), 1058.pt_PT
dc.identifier.doi10.3390/pr8091058pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4688
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDemarcated Region of Madeirapt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectStrainpt_PT
dc.subjectMolecular identificationpt_PT
dc.subjectVolatile organic metabolitespt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleMolecular identification and VOMs characterization of Saccharomyces cerevisiae strains isolated from Madeira region winery environmentspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1058pt_PT
oaire.citation.titleProcessespt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMartinez Castillo
person.familyNameda Silva
person.familyNameCâmara
person.familyNameKhadem
person.givenNameMariangie
person.givenNameEmanuel Marques
person.givenNameJosé
person.givenNameMahnaz
person.identifierG-3003-2013
person.identifier.ciencia-idE219-C141-03A5
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0003-2404-8407
person.identifier.orcid0000-0002-2505-1045
person.identifier.orcid0000-0003-1965-3151
person.identifier.orcid0000-0003-0259-1126
person.identifier.scopus-author-id10140393000
person.identifier.scopus-author-id6701439240
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication216a2d69-8188-4d9f-8c12-adb4ba565146
relation.isAuthorOfPublication21d8a5a0-c756-4057-994e-6aba453ce764
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublicationd3e7023b-4302-47cc-9ee5-d071a218cd9d
relation.isAuthorOfPublication.latestForDiscoverye10d78be-e547-4d25-92b5-06a997ed78da
relation.isProjectOfPublicatione30e13d9-be9a-4f34-91ea-a1682abce74e
relation.isProjectOfPublicatione64ad36a-83b5-467c-bbb3-449ffc0662f2
relation.isProjectOfPublication.latestForDiscoverye30e13d9-be9a-4f34-91ea-a1682abce74e

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Molecular Identification and VOMs Characterization of Saccharomyces cerevisiae Strains Isolated from Madeira Region Winery Environments.pdf
Size:
1.52 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artigos em revistas internacionais