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Publication
Wolbachia (Alphaproteobacteria: Rickettsiales) infections in isolated aphid populations from oceanic islands of the azores archipelago: revisiting the supergroups M and N
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Advisor(s)
Abstract(s)
Aphids (Hemiptera: Aphididae) have provided a suitable model to study endosymbionts, their community, and
dynamics since the discovery of the obligate endosymbiont Buchnera aphidicola in these organisms. In previous
studies, Wolbachia was found in some aphid species. In the present study, we report the prevalence of Wolbachia
in aphids sampled from a geographically isolated region (Azores Islands), aiming at a better understanding and
characterization of the two newly reported supergroups, M and N. The description of the supergroup M was based
on 16S rRNA as well as some protein-coding genes. However, the assignment of the supergroup N was according to
16S rRNA gene sequences of a very few samples. We collected aphid samples and performed phylogenetic analysis
of 16S rRNA gene as well as four protein-coding genes (gatB, ftsZ, coxA, and hcpA). The results demonstrate that
the 16S rRNA gene data can unambiguously assign the strain supergroup and that the two supergroups, N and M,
are equally prevalent in Azorean aphids. The available sequence data for the protein-coding markers can identify
supergroup M but the status of supergroup N is inconclusive, requiring further studies. The data suggest that
horizontal transmission of Wolbachia (Hertig and Wolbach) between two phylogenetically distant aphid species
cohabiting the same plant host.
Description
Keywords
Endosymbiosis Phylogenetic analysis Protein-coding genes and 16S rRNA genes . Faculdade de Ciências da Vida
Citation
Moreira, M., Aguiar, A. M., Bourtzis, K., Latorre, A., & Khadem, M. (2019). Wolbachia (Alphaproteobacteria: Rickettsiales) infections in isolated aphid populations from oceanic islands of the azores archipelago: revisiting the supergroups M and N. Environmental entomology, 48(2), 326-334. DOI: 10.1093/ee/nvy189
Publisher
Entomological Society of America