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Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal

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dc.contributor.authorRagonezi, Carla
dc.contributor.authorCaldeira, A. Teresa
dc.contributor.authorMartins, M. Rosário
dc.contributor.authorSalvador, Cátia
dc.contributor.authorSantos-Silva, Celeste
dc.contributor.authorGanhão, Elsa
dc.contributor.authorKlimaszewska, Krystyna
dc.contributor.authorZavattieri, Maria Amely
dc.date.accessioned2022-02-08T16:11:53Z
dc.date.available2022-02-08T16:11:53Z
dc.date.issued2013
dc.description.abstractStone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have benefi cial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were col lected, pure cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes. To confirm the identity of the collected material, ITS amplification was applied using the pure cultures derived from the sporo carps. Additionally, a molecular profile using PCR based genomic fingerprinting comparison was executed with other genera of Basidiomycetes and Ascomycetes. Our results showed the effective ness of the techniques used to amplify DNA polymorphic sequences, which enhances the characte rization of the genetic profile of ECM fungi and also provides an option to verify the fungus identity at any stage of plant mycorrhization.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRagonezi, C., Caldeira, A. T., Rosário Martins, M., Salvador, C., Santos-Silva, C., Ganhão, E., ... & Zavattieri, A. (2013). Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal. Brazilian Journal of Microbiology, 44(2), 663-670.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4064
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSociedade Brasileira de Microbiologiapt_PT
dc.relationStrategic Project - UI 115 - 2011-2012
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectPisolithus arhizuspt_PT
dc.subjectLactarius deliciosuspt_PT
dc.subjectPinus pineapt_PT
dc.subjectM13-PCRpt_PT
dc.subjectITSpt_PT
dc.subject.pt_PT
dc.subjectEscola Superior de Tecnologias e Gestãopt_PT
dc.titleMolecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 115 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-CFL%2F71437%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FAGR%2FUI0115%2F2011/PT
oaire.citation.conferencePlaceBrasilpt_PT
oaire.citation.endPage670pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage663pt_PT
oaire.citation.titleBrazilian Journal of Microbiologypt_PT
oaire.citation.volume44pt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6820 - DCRRNI ID
person.familyNameRagonezi
person.familyNameZavattieri
person.givenNameCarla
person.givenNameMaria Amely
person.identifier.ciencia-idFC12-D3F0-EF3D
person.identifier.orcid0000-0002-1822-5473
person.identifier.orcid0000-0001-9640-8227
person.identifier.scopus-author-id36460033800
person.identifier.scopus-author-id35486257300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication4a188050-e705-41e2-af9c-2a67aecad273
relation.isAuthorOfPublication.latestForDiscoverya51cc988-63ce-40e5-9b71-a27b1eadc937
relation.isProjectOfPublication6d373255-42e5-4099-9487-7e2aa0f611e8
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