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Extracellular polymeric substances produced by the Thermophilic Cyanobacterium Gloeocapsa gelatinosa: characterization and assessment of their antioxidant and metal-chelating activities

2022Journal article Open access
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dc.contributor.authorGongi, Wejdene
dc.contributor.authorGomez Pinchetti, Juan Luis
dc.contributor.authorCordeiro, Nereida
dc.contributor.authorOuada, Hatem Ben
dc.date.accessioned2022-07-05T13:18:33Z
dc.date.available2022-07-05T13:18:33Z
dc.date.issued2022
dc.description.abstractCyanobacteria, particularly thermophilic strains, represent an important potential source of EPSs, harboring structural complexity that predicts diverse and specific bioactive potential. The ther mophilic cyanobacteria Gloeocapsa gelatinosa, isolated from a natural hot source in Ain Echfa (Tunisia), was cultivated in a cylindrical reactor, and the production of biomass and EPSs was investigated. Results revealed that the strain is amongst the most efficient EPSs producers (0.89 g L−1 ) and that EPSs production was not correlated with the growth phase. EPSs were sulfated heteropolysaccharides containing carbohydrates (70%) based on nine different monosaccharides, mainly mannose (22%), and with the presence of two uronic acids. EPSs were formed by two polymers moieties with a molecular weight of 598.3 ± 7.2 and 67.2 ± 4.4 kDa. They are thermostable in temperatures exceeding 100 ◦C and have an anionic nature (zeta potential of −40 ± 2 mV). Atomic force microscopy showed that EPSs formed multimodal lumps with 88 nm maximum height. EPSs presented high water holding capacity (70.29 ± 2.36%) and solubility index (97.43 ± 1.24%), and a strong bivalent metal sorption capacity especially for Cu2+ (91.20 ± 1.25%) and Fe2+ (75.51 ± 0.71%). The antioxidant activity of G. gelatinosa EPSs was investigated using four methods: the β-carotene-bleaching activity, DPPH assays, iron-reducing activity, and metal-chelating activity. EPS has shown high potential as free radicals’ scavenger, with an IC50 on DPPH (0.2 g L−1 ) three-fold lower than ascorbic acid (0.6 g L −1 ) and as a metal chelating activity (IC50 = 0.4 g L−1 ) significantly lower than EDTA. The obtained results allow further exploration of the thermophilic G. gelatinosa for several biotechnological and industrial applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGongi, W., Gomez Pinchetti, J. L., Cordeiro, N., & Ouada, H. B. (2022). Extracellular polymeric substances produced by the Thermophilic Cyanobacterium Gloeocapsa gelatinosa: characterization and assessment of their antioxidant and metal-chelating activities. Marine Drugs, 20(4), 227.pt_PT
dc.identifier.doi10.3390/md20040227pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4363
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGloeocapsa gelatinosapt_PT
dc.subjectExtracellular polymeric substancespt_PT
dc.subjectPhysicochemical characterizationpt_PT
dc.subjectFunctional propertiespt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectExtremophilicpt_PT
dc.subjectCyanobacteriapt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleExtracellular polymeric substances produced by the Thermophilic Cyanobacterium Gloeocapsa gelatinosa: characterization and assessment of their antioxidant and metal-chelating activitiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue4pt_PT
oaire.citation.startPage227pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume20pt_PT
person.familyNamecordeiro
person.givenNamenereida
person.identifier1612636
person.identifier.ciencia-id521A-0F62-EA39
person.identifier.orcid0000-0001-6006-3415
person.identifier.ridO-2253-2019
person.identifier.scopus-author-id7004319456
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3d02b657-c48c-4f56-813a-1284b81960f3
relation.isAuthorOfPublication.latestForDiscovery3d02b657-c48c-4f56-813a-1284b81960f3

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