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Gene delivery into mesenchymal stem cells: a biomimetic approach using RGD nanoclusters based on poly(amidoamine) dendrimers

2011-02-14Journal article Restricted access
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dc.contributor.authorPandita, Deepti
dc.contributor.authorSantos, José L.
dc.contributor.authorRodrigues, João
dc.contributor.authorPêgo, Ana P.
dc.contributor.authorGranja, Pedro L.
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-06-18T11:18:02Z
dc.date.available2019-06-18T11:18:02Z
dc.date.issued2011-02-14
dc.description.abstractPoly(amidoamine) dendrimers (generations 5 and 6) with amine termini were conjugated with peptides containing the arginine-glycine-aspartic acid (RGD) sequence having in view their application as gene delivery vectors. The idea behind the work was to take advantage of the cationic nature of dendrimers and of the integrin targeting capabilities of the RGD motif to improve gene delivery. Dendrimers were used as scaffolds for RGD clustering and, by controlling the number of peptides (4, 8, and 16) linked to each dendrimer, it was possible to evaluate the effect of RGD density on the gene delivery process. The new vectors were characterized in respect to their ability to neutralize and compact plasmid DNA (pDNA). The complexes formed by the vectors and pDNA were studied concerning their size, zeta potential, capacity of being internalized by cells and ability of transferring genes. Transfection efficiency was analyzed, first, by using a pDNA encoding for Enhanced Green Fluorescent Protein and Firefly Luciferase and, second, by using a pDNA encoding for Bone Morphogenetic Protein-2. Gene expression in mesenchymal stem cells was enhanced using the new vectors in comparison to native dendrimers and was shown to be dependent on the electrostatic interaction established between the dendrimer moiety and the cell surface, as well as on the RGD density of nanoclusters. The use of dendrimer scaffolds for RGD cluster formation is a new approach that can be extended beyond gene delivery applications, whenever RGD clustering is important for modulating cellular responses.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPandita, D., Santos, J. L., Rodrigues, J., Pêgo, A. P., Granja, P. L., & Tomás, H. (2011). Gene delivery into mesenchymal stem cells: a biomimetic approach using RGD nanoclusters based on poly (amidoamine) dendrimers. Biomacromolecules, 12(2), 472-481.pt_PT
dc.identifier.doi10.1021/bm1012647pt_PT
dc.identifier.issn1525-7797
dc.identifier.urihttp://hdl.handle.net/10400.13/2404
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationBONE TISSUE ENGINEERING VIA LOCAL GENE DELIVERY
dc.subjectAnimalspt_PT
dc.subjectCell survivalpt_PT
dc.subjectDendrimerspt_PT
dc.subjectMalept_PT
dc.subjectMesenchymal stem cellspt_PT
dc.subjectMolecular structurept_PT
dc.subjectNanostructurespt_PT
dc.subjectOligopeptidespt_PT
dc.subjectPolyaminespt_PT
dc.subjectRatspt_PT
dc.subjectRats, Wistarpt_PT
dc.subjectStructure-activity relationshippt_PT
dc.subjectBiomimeticspt_PT
dc.subjectGene transfer techniquespt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleGene delivery into mesenchymal stem cells: a biomimetic approach using RGD nanoclusters based on poly(amidoamine) dendrimerspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBONE TISSUE ENGINEERING VIA LOCAL GENE DELIVERY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BEB%2F71161%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI-2010/SFRH%2FBD%2F19450%2F2004/PT
oaire.citation.endPage481pt_PT
oaire.citation.startPage472pt_PT
oaire.citation.titleBiomacromoleculespt_PT
oaire.citation.volume12(2)pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamPOCI-2010
person.familyNameRodrigues
person.familyNamePêgo
person.familyNameTomás
person.givenNameJoão
person.givenNameAna Paula
person.givenNameHelena
person.identifier164495
person.identifier556975
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id6114-92F0-E64C
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0001-5169-328X
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridB-6816-2008
person.identifier.ridC-6942-2012
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id56053889300
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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