Effect of time and temperature on vitamin C stability in horticultural extracts. UHPLC-PDA vs iodometric titration as analytical methods

Spínola, Vítor; Mendes, Berta; Câmara, José S.; Castilho, Paula C.

http://hdl.handle.net/10400.13/2478

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Abstract(s)

Several fruits and vegetables from Madeira Island (Portugal) were evaluated by two analytical methods for their total vitamin C content (l-ascorbic acid, l-AA and dehydroascorbic acid, DHAA). DHAA was determined indirectly with DL-1,4-dithiotreitol (DTT) applied as a pre-column reductant. Ultra high performance liquid chromatography coupled to photodiode array (UHPLC-PDA) determinations were compared with l-AA content obtained by a classic iodometric titration method. The stability of vitamin C in horticultural extracts stored at different temperatures was also investigated. Red peppers represented the better source of vitamin C followed by green peppers and papayas. Passion fruits and cherimoyas were the analyzed foodstuffs with lowest vitamin C content. Both analytical methods were suitable for l-AA analysis in various food commodities, the UHPLC-PDA technique being preferred due to its advantages of selectivity, speed and accuracy. The degradation study showed that horticultural extracts were stable at least 24 h at 4 °C and during 4 weeks when stored at −80 °C.

Keywords

Ascorbic acid Dehydroascorbic acid UHPLC Iodometric titration Fruits and vegetables Stability . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira

URI

http://hdl.handle.net/10400.13/2478

Citation

Spínola, V., Mendes, B., Câmara, J. S., & Castilho, P. C. (2013). Effect of time and temperature on vitamin C stability in horticultural extracts. UHPLC-PDA vs iodometric titration as analytical methods. LWT-Food Science and Technology, 50(2), 489-495.