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A powerful analytical strategy based on QuEChERS-dispersive solid-phase extraction combined with ultrahigh pressure liquid chromatography for evaluating the effect of elicitors on biosynthesis of trans-resveratrol in grapes

2015Journal article Restricted access
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dc.contributor.authorRuiz-García, Yolanda
dc.contributor.authorSilva, Catarina L.
dc.contributor.authorGómez-Plaza, Encarna
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-23T13:10:18Z
dc.date.available2019-07-23T13:10:18Z
dc.date.issued2015
dc.description.abstractA powerful methodological approach based on modified quick, easy, cheap effective, rugged, and safe (QuEChERS) extraction technique, followed by cleanup dispersive solid-phase extraction (dSPE) and combined with ultrahigh pressure liquid chromatography (UHPLC), is presented in this paper, for the rapid determination of the health-promoting phytoalexin, trans-resveratrol, in grapes. This is the first time, to our knowledge, that the combination QuEChERS-dSPE/UHPLC-PDA has been used for trans-resveratrol quantification in grapes. The method has been validated according to European Union Decision 2002/657/EC, in terms of selectivity, linearity, sensitivity, instrument LOD and method LOQ, accuracy, precision, extraction efficiency, and interference assessment. Validation experiments revealed sufficient linearity (R 2 = 0.9931) within the established concentration range confirmed by Mandel’s fitting test. The sensitivity was good with method detection limit (LOD) of 0.003 μg/mL and quantification limit (LOQ) of 0.010 μg/mL. Optimum QuEChERS-dSPE/UHPLC-PDA conditions led to recoveries of the target analyte in grape samples ranging between 75.1 and 99.7 % and precisions in the 0.9–4 % range (RSD, n = 18). The method also afforded satisfactory results in terms of extraction efficiency and interference assessment and provides a valuable and promising tool for determination of trans-resveratrol in grapes with a high resolution within 8 min and a total analysis time of 11 min. The validated methodology was applied to evaluate the effect of the use of elicitors, namely benzothiadiazole and methyl jasmonate, in trans-resveratrol biosynthesis on Vitis vinifera Monastrell grapes. The results obtained revealed an increase of trans-resveratrol level of 2-fold for grapes treated with benzothiadiazole and 3.5-fold for grapes treated with methyl jasmonate.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRuiz-García, Y., Silva, C. L., Gómez-Plaza, E., & Câmara, J. S. (2016). A powerful analytical strategy based on QuEChERS-dispersive solid-phase extraction combined with ultrahigh pressure liquid chromatography for evaluating the effect of elicitors on biosynthesis of trans-resveratrol in grapes. Food analytical methods, 9(3), 670-679.pt_PT
dc.identifier.doi10.1007/s12161-015-0227-2pt_PT
dc.identifier.issn1936-9751
dc.identifier.urihttp://hdl.handle.net/10400.13/2500
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.subjecttrans-Resveratrolpt_PT
dc.subjectGrapespt_PT
dc.subjectElicitorspt_PT
dc.subjectUHPLC-PDApt_PT
dc.subjectdSPE-QuEChERSpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleA powerful analytical strategy based on QuEChERS-dispersive solid-phase extraction combined with ultrahigh pressure liquid chromatography for evaluating the effect of elicitors on biosynthesis of trans-resveratrol in grapespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.citation.endPage679pt_PT
oaire.citation.startPage670pt_PT
oaire.citation.titleFood Analytical Methodspt_PT
oaire.citation.volume9(3)pt_PT
oaire.fundingStream5876
person.familyNameSousa Luís
person.familyNameCâmara
person.givenNameCatarina Grace
person.givenNameJosé
person.identifierC-1300-2019
person.identifierG-3003-2013
person.identifier.ciencia-id9813-3B88-BA8D
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-3018-3165
person.identifier.orcid0000-0003-1965-3151
person.identifier.scopus-author-id57194492726
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2853e6b6-0fb5-454c-aedb-05528b76c484
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscoverye10d78be-e547-4d25-92b5-06a997ed78da
relation.isProjectOfPublicationec6bb904-b852-4300-a638-266ee44773f0
relation.isProjectOfPublication.latestForDiscoveryec6bb904-b852-4300-a638-266ee44773f0

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