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Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study

2022Journal article Open access
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dc.contributor.authorNesic, Maja D.
dc.contributor.authorDucic, Tanja
dc.contributor.authorAlgarra, Manuel
dc.contributor.authorPopović, Iva
dc.contributor.authorStepić, Milutin
dc.contributor.authorGonçalves, Mara
dc.contributor.authorPetkovic, Marijana
dc.date.accessioned2023-06-19T10:31:24Z
dc.date.available2023-06-19T10:31:24Z
dc.date.issued2022
dc.description.abstractIn the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive un derstanding of the effect of the drug [Ru(η 5 -Cp)(PPh3 )2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane–water interface region.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNeši´c, M.D.; Duˇci´c, T.; Algarra, M.; Popovi´c, I.; Stepi´c, M.; Gonçalves, M.; Petkovi´c, M. Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study. Cancers 2022, 14, 1182. https://doi.org/10.3390/ cancers14051182pt_PT
dc.identifier.doi10.3390/cancers14051182pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/5233
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnticancer Ru metallodrugpt_PT
dc.subjectCarbon dotspt_PT
dc.subjectN-doped carbon dotspt_PT
dc.subjectDrug nanocarrierspt_PT
dc.subjectLipidspt_PT
dc.subjectMALDI TOF MSpt_PT
dc.subjectSR-FTIR spectroscopypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleLipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.citation.issue5pt_PT
oaire.citation.startPage1182pt_PT
oaire.citation.titleCancerspt_PT
oaire.citation.volume14pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameNesic
person.familyNameDucic
person.familyNameAlgarra
person.familyNameGonçalves
person.familyNamePetkovic
person.givenNameMaja
person.givenNameTanja
person.givenNameManuel
person.givenNameMara
person.givenNameMarijana
person.identifierC-5257-2008
person.identifier.ciencia-id3011-173A-EBBC
person.identifier.ciencia-idF911-74AF-6932
person.identifier.ciencia-id6010-1863-4B11
person.identifier.ciencia-idAD15-C921-5954
person.identifier.orcid0000-0002-9167-6553
person.identifier.orcid0000-0003-2389-3887
person.identifier.orcid0000-0003-2410-8430
person.identifier.orcid0000-0002-0332-3548
person.identifier.orcid0000-0002-8354-995X
person.identifier.ridF-5107-2019
person.identifier.ridH-5567-2016
person.identifier.scopus-author-id56559929100
person.identifier.scopus-author-id6602455238
person.identifier.scopus-author-id55509603000
person.identifier.scopus-author-id7006986199
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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