Repository logo
 
Publication

New insights into the blue intrinsic fluorescence of oxidized PAMAM dendrimers considering their use as bionanomaterials

2020Journal article Open access
Simple item page
dc.contributor.authorCamacho, Cláudia S.
dc.contributor.authorUrgellés, Marta
dc.contributor.authorTomás, Helena
dc.contributor.authorLahoz, Fernando
dc.contributor.authorRodrigues, João
dc.date.accessioned2022-02-18T14:49:31Z
dc.date.available2022-02-18T14:49:31Z
dc.date.issued2020
dc.description.abstractLike other bionanomaterials, dendrimers are usually labelled with fluorescent compounds in order to be optically detected within cells. However, this process can interfere with their biological properties, so it is crucial to find other solutions for their traceability. Here, the blue intrinsic fluorescence of amine terminated poly(amidoamine) (PAMAM) dendrimers was enhanced using oxidative treatment with ammonium persulfate (APS). The effects of dendrimer generation (G3, G4, and G5) and pH on the spectroscopic behavior of both pristine and APS-treated PAMAM dendrimers were studied in aqueous solution. Overall, the results pointed out that there are at least two types of emitting electron-rich hetero-atomic sub-luminophores (HASLs) confined within the dendrimer scaffold that have very close maximum emission wavelengths and whose emission properties strongly depend on pH. The APS treatment significantly enhanced the fluorescence intensity by leading to the protonation of the interior of the dendrimer. However, fluorescence intensity was not only dependent on the number of HASLs in the dendrimer scaffold (i.e., on dendrimer generation), but also on the rigidification suffered by the dendrimer due to the acidic environment (at low pH values, APS-treated G4 was indeed the most emissive species). Moreover, photoluminescence studies with lyophilized samples were also conducted, which confirmed the coexistence of more than one type of HASLs emitting in the dendrimer structure. The APS treatment affected these HASLs to a different extent. Time-resolved fluorescence experiments always showed higher average lifetimes of HASLs for APS-treated dendrimers than for pristine ones, in accordance with the fluorescence intensity results. On the other hand, the fraction and lifetimes of HASLs in APS-treated dendrimers were similar in solution and the lyophilized form. This behaviour was different for the pristine dendrimers that presented increased luminescence upon aggregation. Finally, the highly emissive oxidized dendrimers were shown not only to be much less cytotoxic and hemotoxic than pristine dendrimers but also to be detectable inside cells upon excitation with UV light.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCamacho, C. S., Urgellés, M., Tomás, H., Lahoz, F., & Rodrigues, J. (2020). New insights into the blue intrinsic fluorescence of oxidized PAMAM dendrimers considering their use as bionanomaterials. Journal of Materials Chemistry B, 8(45), 10314-10326.pt_PT
dc.identifier.doi10.1039/D0TB01871Fpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4094
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBionanomaterialspt_PT
dc.subjectDendrimerspt_PT
dc.subjectPAMAMpt_PT
dc.subjectOxidized PAMAM dendrimerspt_PT
dc.subjectBlue intrinsic fluorescencept_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleNew insights into the blue intrinsic fluorescence of oxidized PAMAM dendrimers considering their use as bionanomaterialspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.citation.endPage10326pt_PT
oaire.citation.issue45pt_PT
oaire.citation.startPage10314pt_PT
oaire.citation.titleJournal of Materials Chemistry Bpt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCamacho
person.familyNameTomás
person.familyNameLahoz
person.familyNameRodrigues
person.givenNameCláudia Sofia
person.givenNameHelena
person.givenNameFernando
person.givenNameJoão
person.identifier556975
person.identifier.ciencia-id6C1E-7D62-56D1
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0001-8008-1696
person.identifier.orcid0000-0003-4552-1953
person.identifier.ridE-5991-2010
person.identifier.ridS-1251-2017
person.identifier.ridB-6816-2008
person.identifier.scopus-author-id6508104177
person.identifier.scopus-author-id55161203400
person.identifier.scopus-author-id9233278800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationaa717a69-56df-4662-ac36-690c12a81f11
relation.isAuthorOfPublication3a747114-6ab9-454b-985b-00cbf792e273
relation.isAuthorOfPublication6abadeea-300d-41a2-b37e-a0f2fbb03c52
relation.isAuthorOfPublication53031fca-b2e7-4ce4-bda9-61e486da4547
relation.isAuthorOfPublication.latestForDiscovery6abadeea-300d-41a2-b37e-a0f2fbb03c52
relation.isProjectOfPublicatione30e13d9-be9a-4f34-91ea-a1682abce74e
relation.isProjectOfPublicatione64ad36a-83b5-467c-bbb3-449ffc0662f2
relation.isProjectOfPublication.latestForDiscoverye64ad36a-83b5-467c-bbb3-449ffc0662f2

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
New insights into the blue intrinsic fluorescence of oxidized PAMAM dendrimers considering their use as bionanomaterials.pdf
Size:
5.43 MB
Format:
Adobe Portable Document Format
Download

Collections

Artigos em revistas internacionais