Ultrastructural and molecular characterization of Glugea serranus n. sp., a microsporidian infecting the blacktail comber, Serranus atricauda (Teleostei: Serranidae), in the Madeira Archipelago (Portugal)

Casal, Graça; Rocha, Sónia; Costa, Graça; Al-Quraishy, Saleh; Azevedo, Carlos

http://hdl.handle.net/10400.13/4782

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Abstract(s)

A new microsporidian infecting the connective tis sue of the coelomic cavity of the blacktail comber Serranus atricauda, in the Madeira Archipelago (Portugal), is described on the basis of morphological, ultrastructural, and molecular features. The microsporidian formed large whitish xenomas adhering to the peritoneal visceral organs of the host. Each xenoma consisted of a single hypertrophic cell, in the cyto plasm of which mature spores proliferated within parasitophorous vacuoles surrounded by numerous collagen fibers. Mature spores were ellipsoidal and uninucleated, mea suring an average of 6.5 ± 0.5 μm in length and 3.4 ± 0.6 μm in width. The anchoring disk of the polar filament was subter minal, laterally shifted from the anterior pole of the spore. The isofilar polar filament coiled in 18–19 turns, forming two rows that surrounded the posterior vacuole. The latter occupied about one third of the spore length. The polaroplast surround ing the apical and uncoiled portion of the polar filament displayed two distinct regions: a lamellar region and an electron-dense globule. Molecular analysis of the rRNA genes, including the internal transcribed spacer region, and phylogenetic analysis using maximum likelihood and neigh bor joining demonstrated that this microsporidian parasite clustered with some Glugea species. Based on the differences found both at the morphological and molecular levels, to other members of the genus Glugea, the microsporidian infecting the blacktail comber is considered a new species, thus named Glugea serranus n. sp.

Keywords

Fish parasite Microsporidia Fine structure rRNA genes . Faculdade de Ciências da Vida

URI

http://hdl.handle.net/10400.13/4782

Citation

Casal, G., Rocha, S., Costa, G. et al. Ultrastructural and molecular characterization of Glugea serranus n. sp., a microsporidian infecting the blacktail comber, Serranus atricauda (Teleostei: Serranidae), in the Madeira Archipelago (Portugal). Parasitol Res 115, 3963–3972 (2016). https://doi.org/10.1007/s00436-016-5162-7