Artigos em revistas internacionais
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Browsing Artigos em revistas internacionais by Field of Science and Technology (FOS) "Ciências Médicas::Outras Ciências Médicas"
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- Editorial: Interaction of microbiota and metabolomic disordersPublication . Pereira, Jorge A. M.; Augusto Machado Pereira, Jorge
- MALDI-TOF MS: a promising analytical approach to cancer diagnostics and monitoringPublication . Sousa, Patrícia; Silva, Laurentina; Luís, Catarina; Câmara, José S.; Perestrelo, Rosa; Sousa Luís, Catarina Grace; Câmara, José; Perestrelo, RosaCancer remains the second most common cause of death after cardiovascular diseases, accounting for nearly 10 million deaths in 2020. Although the incidence of cancer increases considerably with age, the cancer burden can also be reduced and have a high chance of cure through early detection, appropriate treatment, and care of patients. The development of high-throughput analytical approaches, like matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), contributes to identifying a pool of proteins/peptides as putative biomarkers for the early detection, diagnosis, and tumor progression. The purpose of the current review is to present an updated outline of recent proteome/peptidome research to establish putative cancer biomarkers using MALDI-TOF MS and highlight the applicability of statistical analysis in the oncology field. The pros and cons of MALDI-TOF MS application on cancer diagnostics and monitoring will be discussed, as well as compared with tandem mass spectrometry (MS/MS)-based proteomics (e.g., liquid chromatography–tandem mass spectrometry). In addition, pre-analytical (e.g., sample quality control) and analytical (e.g., sample pre-treatment, instrumental analytical conditions) properties that influence the robustness of MALDI-TOF MS data will be also discussed.
- Prevalence of autism spectrum disorder in children in the Azores Islands (Portugal): sociodemographic and clinical profilePublication . Conde, Ana Rita; Mota, Pilar; Botelho, Tânia; Caldeira, Suzana; Rego, Isabel; Silva, Osvaldo; Sousa, Áurea Sandra Toledo de; Freitas, Carina; De Barros Freitas, Carina Patrícia; Freitas, CarinaAbstract Prevalence studies are essential to provide objective indicators about Autism Spectrum Disorder (ASD) and are a source of information for public policies. This study aimed to estimate the prevalence of ASD among children in the Azores Islands (Portugal). Administrative data on the number of children with a proven diagnosis of ASD were collected from all schools. Parents completed a questionnaire about the child’s sociodemographic characteristics, the diagnostic process, and clinical history. The overall prevalence of ASD in the Azores region was 9.92 per 1000 children, approximately equivalent to 1% (0.99). The prevalence of ASD in the Azores seems to be higher when compared with the global rate in Portugal, as well as with other regions of Europe. There appears to be a concentration of children with ASD in the region, and the results appear to support the hypothesis of a heritable predisposition to ASD.
- Understanding the tolerance of different strains of human pathogenic bacteria to acidic environmentsPublication . Figueira, Priscilla Porto; Câmara, José S.; Vigário, Ana M.; Pereira, Jorge A. M.; Porto-Figueira, Priscilla; Câmara, José; Augusto Machado Pereira, JorgeIt is known that many bacteria are susceptible to low pH, but it is less clear how the acid nature influences this susceptibility. The objective of this work was to evaluate the susceptibility of selected bacteria to different low pH conditions and to understand if their tolerance is affected by the type of acid (organic vs inorganic) in the media acidification. Strains of different species of human pathogenic bacteria were cultivated at different acidic conditions, using organic or inorganic acids. Overall, we verified that tolerance to acidic conditions is dependent on the type of acid used to acidify the growing medium, organic or inorganic. The strains analysed, although having different tolerance to low pH, were shown to be more susceptible to organic than inorganic acids. This result shows that beyond the pH of the media, the nature of the acid affects bacterial growth. Such a result has consequences in the evaluation of the efficiency of an antibacterial extract, which should take into consideration the differential susceptibility of bacteria to low pHs obtained using organic or inorganic acids.