Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex

Nesic, Maja D.; Ducic, Tanja; Gonçalves, Mara; Stepić, Milutin; Algarra, Manuel; Soto, Juan; Gemović, Branislava; Bandosz, Teresa J.; Petkovic, Marijana

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Resumo(s)

Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respec tively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination.

Palavras-chave

Anticancer photodynamic therapy Biochemical changes N-doped carbon dots Carbon dots Ru-metallodrug . Faculdade de Ciências Exatas e da Engenharia

URI

http://hdl.handle.net/10400.13/5234

Citação

Nešić, M. D., Dučić, T., Gonçalves, M., Stepić, M., Algarra, M., Soto, J., ... & Petković, M. (2022). Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex. Chemico-Biological Interactions, 360, 109950.