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Amphiphilic polymer-mediated formation of laponite-based nanohybrids with robust stability and pH sensitivity for anticancer drug delivery

2014Journal article Restricted access
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dc.contributor.authorWang, Guoying
dc.contributor.authorMaciel, Dina
dc.contributor.authorWu, Yilun
dc.contributor.authorRodrigues, João
dc.contributor.authorShi, Xiangyang
dc.contributor.authorYuan, Yuan
dc.contributor.authorLiu, Changsheng
dc.contributor.authorTomás, Helena
dc.contributor.authorLi, Yulin
dc.date.accessioned2019-06-21T11:14:26Z
dc.date.available2019-06-21T11:14:26Z
dc.date.issued2014
dc.description.abstractThe development of pH-sensitive drug delivery nanosystems that present a low drug release at the physiological pH and are able to increase the extent of the release at a lower pH value (like those existent in the interstitial space of solid tumors (pH 6.5) and in the intracellular endolysosomal compartments (pH 5.0)) is very important for an efficient and safe cancer therapy. Laponite (LP) is a synthetic silicate nanoparticle with a nanodisk structure (25 nm in diameter and 0.92 nm in thickness) and negative-charged surface, which can be used for the encapsulation of doxorubicin (DOX, a cationic drug) through electrostatic interactions and exhibit good pH sensitivity in drug delivery. However, the colloidal instability of LP still limits its potential clinical applications. In this study, we demonstrate an elegant strategy to develop stable Laponite-based nanohybrids through the functionalization of its surface with an amphiphile PEG-PLA copolymer by a self-assembly process. The hydrophobic block of PEG-PLA acts as an anchor that binds to the surface of drug-loaded LP nanodisks, maintaining the core structure, whereas the hydrophilic PEG part serves as a protective stealth shell that improves the whole stability of the nanohybrids under physiological conditions. The resulting nanocarriers can effectively load the DOX drug (the encapsulation efficiency is 85%), and display a pH-enhanced drug release behavior in a sustained way. In vitro biological evaluation indicated that the DOX-loaded nanocarriers can be effectively internalized by CAL-72 cells (an osteosarcoma cell line), and exhibit a remarkable higher anticancer cytotoxicity than free DOX. The merits of Laponite/PEG-PLA nanohybrids, such as good cytocompatibility, excellent physiological stability, sustained pH-responsive release properties, and improved anticancer activity, make them a promising platform for the delivery of other therapeutic agents beyond DOX.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationWang, G., Maciel, D., Wu, Y., Rodrigues, J., Shi, X., Yuan, Y., ... & Li, Y. (2014). Amphiphilic polymer-mediated formation of laponite-based nanohybrids with robust stability and pH sensitivity for anticancer drug delivery. ACS applied materials & interfaces, 6(19), 16687-16695.pt_PT
dc.identifier.doi10.1021/am5032874pt_PT
dc.identifier.issn1944-8244
dc.identifier.urihttp://hdl.handle.net/10400.13/2416
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.relationSelf-assembled nanoparticles based on PEG-PLA-dendrimer building blocks for dual gene/drug delivery
dc.subjectAnimalspt_PT
dc.subjectAntineoplastic agentspt_PT
dc.subjectCell deathpt_PT
dc.subjectTumorpt_PT
dc.subjectCell shapept_PT
dc.subjectCell survivalpt_PT
dc.subjectDoxorubicinpt_PT
dc.subjectDrug liberationpt_PT
dc.subjectEndocytosispt_PT
dc.subjectHumanspt_PT
dc.subjectHydrogen-ion concentrationpt_PT
dc.subjectMicept_PT
dc.subjectFluorescence microscopypt_PT
dc.subjectNIH 3T3 cellspt_PT
dc.subjectNanoparticlespt_PT
dc.subjectPolymerspt_PT
dc.subjectSilicatespt_PT
dc.subjectUltraviolet spectrophotometrypt_PT
dc.subjectFourier transform infrared spectroscopypt_PT
dc.subjectSurface-active agentspt_PT
dc.subjectDrug delivery systemspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleAmphiphilic polymer-mediated formation of laponite-based nanohybrids with robust stability and pH sensitivity for anticancer drug deliverypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardTitleSelf-assembled nanoparticles based on PEG-PLA-dendrimer building blocks for dual gene/drug delivery
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-NAN%2F116788%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-NAN%2F112428%2F2009/PT
oaire.citation.endPage16695pt_PT
oaire.citation.startPage16687pt_PT
oaire.citation.titleACS Applied Materials and Interfacespt_PT
oaire.citation.volume6(19)pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameMaciel
person.familyNameRodrigues
person.familyNameShi
person.familyNameTomás
person.familyNameLi
person.givenNameDina
person.givenNameJoão
person.givenNameXiangyang
person.givenNameHelena
person.givenNameYulin
person.identifier556975
person.identifier.ciencia-id211C-6048-FB1A
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-idBA11-1437-B948
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0001-8684-6100
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0001-6785-6645
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0001-5569-1038
person.identifier.ridB-6816-2008
person.identifier.ridA-1289-2007
person.identifier.ridE-5991-2010
person.identifier.ridA-4082-2013
person.identifier.scopus-author-id54781586200
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id7402953116
person.identifier.scopus-author-id6508104177
person.identifier.scopus-author-id55718977200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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