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Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira

2005Journal article Open access
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dc.contributor.authorFreitas, I. R. A. de
dc.contributor.authorGanança, F.
dc.contributor.authorSantos, T. M. dos
dc.contributor.authorCarvalho, M. A. A. Pinheiro de
dc.contributor.authorMotto, M.
dc.contributor.authorVieira, M. Clemente
dc.date.accessioned2021-03-19T10:38:30Z
dc.date.available2021-03-19T10:38:30Z
dc.date.issued2005
dc.description.abstractZein polypeptides are a group of proteins that accumulate in maize endosperm during seed development, representing more than 60% of the total endosperm proteins in the mature seeds. To evaluate genotype variability of Madeiran maize germplasm, a biochemical study was conducted based on the prolamins of maize, zeins, extracted from endosperm meal of 43 populations of Zea mays L. maintained in the Germplasm bank of Madeira University along with the inbreed W64A which was used as a polymorphic zein polypeptide standard profile. The zein polymorphism of these 44 maize populations were compared using two different electrophoresis techniques, SDS-PAGE in 15% discontinuous polyacrylamide gel and Acid-PAGE in 10% continuous polyacrylamide gel. SDS-PAGE allowed up to 16 polypeptides to be identified with apparent molecular mass ranging from 28-kDa to 10-kDa. Acid-PAGE allowed up to 20 zein fractions to be identified. The data was submitted to principal component analysis (PCA) and canonical discriminate and similarity analysis. The SDS-PAGE zein polymorphism allowed us to detect 6 groups, assembling all maize populations and explaining 55.32% of all variability. The similarity analysis of zein patterns obtained by Acid-PAGE showed that among regional maize germplasm, 22.5 % of all population seems to be related and have a common ancestor. The ISOP71 seems to be the population more closed to the common ancestor and appears related with the remaining maize populations, excluding the ISOP125.The obtained results and the importance of zein polymorphism in the evaluation of maize germplasm from Madeiran Archipelago are discussedpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFreitas, I. R. A., Ganança, F., Santos, T. M., Carvalho, M. A. A. P., Motto, M., & Vieira, M. C. (2005). Evaluation of maize germplasm based on zein polymorphism from the archipelago of Madeira. Maydica, 50(2), 105-112.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3185
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUnità di Ricerca per la Valorizzazione qualitativa dei cerealipt_PT
dc.relationMorphological, physiological, and molecular characterisation, and conservation of wheat and maize traditional cultivars from the Madeira
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSeed storage proteinspt_PT
dc.subjectZeinpt_PT
dc.subjectSDS-PAGEpt_PT
dc.subjectAcid-PAGEpt_PT
dc.subjectMaize germplasmpt_PT
dc.subjectArchipelago of Madeira (Portugal)pt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleEvaluation of maize germplasm based on zein polymorphism from the archipelago of Madeirapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMorphological, physiological, and molecular characterisation, and conservation of wheat and maize traditional cultivars from the Madeira
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FAGR%2F35003%2F2000/PT
oaire.citation.endPage112pt_PT
oaire.citation.startPage105pt_PT
oaire.citation.titleMaydicapt_PT
oaire.citation.volume50pt_PT
oaire.fundingStreamPOCI
person.familyNameGanança
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameJosé Filipe
person.givenNameMiguel Angelo
person.identifier1218558
person.identifier.ciencia-id4610-6741-6816
person.identifier.orcid0000-0002-8384-6188
person.identifier.orcid0000-0002-5084-870X
person.identifier.ridAEE-0913-2022
person.identifier.scopus-author-id8758577600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery2e433c48-88f8-40a2-b21f-de78b44aab1c
relation.isProjectOfPublication3b776491-3e85-4bbe-9118-726178af8726
relation.isProjectOfPublication.latestForDiscovery3b776491-3e85-4bbe-9118-726178af8726

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