Loading...
Publication
Polimorfismos do gene da ECA e risco de doença coronária numa população portuguesa
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 186.54 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Introdução: A história familiar de doença das
artérias coronárias (DAC) constitui um
poderoso marcador de risco de DAC,
independente dos factores de risco
tradicionais. Poderá ser descodificado
reconhecendo os polimorfismos associados ao
aumento de risco. Têm surgido resultados
contraditórios em relação à ligação entre os
polimorfismos do gene da enzima de conver são da angiotensina (ECA) e o risco de DAC.
Objectivo: Com o presente trabalho
pretendemos avaliar se os polimorfismos do
gene da ECA constituem factor de risco de
doença das artérias coronárias.
Métodos: Estudo caso-controlo, incluindo
517 controlos escolhidos aleatoriamente dos
cadernos eleitorais, sem história sugestiva de
DAC e 301 doentes com história de enfarte
agudo do miocárdio ou doença coronária
confirmada por coronariografia, com pelo
menos 75 % de obstrução de um dos vasos
coronários. Tentou-se que os casos e controlos
não fossem significativamente diferentes em
termos de sexo e idade.
Os polimorfismos dialélicos do gene da ECA
foram tipados por amplificação por PCR. Os
produtos de amplificação eram identificados
em gel de poliacrilamida, por electroforese.
Os dados foram avaliados recorrendo ao SPSS
for Windows,
Background: A family history of coronary heart disease (CHD) is a strong risk marker for the disease, independently of classical risk factors. It could be decoded by recognizing the polymorphisms associated with increased risk. Renin-angiotensin system genes are candidate genes in CHD and the deletion allele of the angiotensin converting enzyme (ACE) has been reported as deleterious. However, there is disagreement as to the role of the insertion/deletion polymorphism of the ACE gene in coronary risk. Aim: To evaluate whether ACE gene polymorphisms constitute a CHD risk factor. Methods: We conducted a population-based case-control study of 301 subjects with a history of myocardial infarction or angiographic evidence of coronary heart disease and 510 age- and gender-matched controls, without CHD, living in a region with high CHD mortality rates. Blood samples were taken, DNA extracted and genotypes determined by the polymerase chain reaction (PCR). Amplification products were identified by agarose gel electrophoresis.
Background: A family history of coronary heart disease (CHD) is a strong risk marker for the disease, independently of classical risk factors. It could be decoded by recognizing the polymorphisms associated with increased risk. Renin-angiotensin system genes are candidate genes in CHD and the deletion allele of the angiotensin converting enzyme (ACE) has been reported as deleterious. However, there is disagreement as to the role of the insertion/deletion polymorphism of the ACE gene in coronary risk. Aim: To evaluate whether ACE gene polymorphisms constitute a CHD risk factor. Methods: We conducted a population-based case-control study of 301 subjects with a history of myocardial infarction or angiographic evidence of coronary heart disease and 510 age- and gender-matched controls, without CHD, living in a region with high CHD mortality rates. Blood samples were taken, DNA extracted and genotypes determined by the polymerase chain reaction (PCR). Amplification products were identified by agarose gel electrophoresis.
Description
Keywords
Doença das artérias coronárias (DAC) Risco de doença coronária Polimorfismos do Gene da ECA Gene da ECA Portugal . Faculdade de Ciências da Vida
Citation
MENDONÇA, I., FREITAS, I. A., SOUSA, C. A., GOMES, S., FARIA, P., DRUMOND, A., ... & COELHO, A. (2004). Polimorfismos do Gene da ECA e Risco de Doença Coronária numa População Portuguesa [104]. Rev Port Cardiol, 23(12), 1593-1601.