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Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery

2021Journal article Open access
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dc.contributor.authorMartins, Ivo
dc.contributor.authorTomás, Helena
dc.contributor.authorLahoz, Fernando
dc.contributor.authorRodrigues, João
dc.date.accessioned2022-02-16T14:44:51Z
dc.date.available2022-06-01T00:30:29Z
dc.date.issued2021
dc.description.abstractABSTRACT: Carbon dots (CDs) and G4-G6 (polyamidoamine)- PAMAM-NH2 dendrimers were self-assembled to produce CDs@ PAMAM nanohybrids for transfection and bioimaging purposes. CDs were synthesized by the hydrothermal method, using ascorbic acid as a starting precursor and characterized by transmission electron microscopy, UV−Vis, and fluorescence (in solution and solid-state) techniques. CDs were electrostatically combined with PAMAM dendrimers at room temperature, and the UV−Vis, fluorescence, and NMR spectroscopies were used to confirm the self-assembly. When compared to pristine CDs, nanohybrids were more photostable, resisting high acidic and basic pH. Moreover, they were considerably internalized by cells, as assessed by flow cytometry and fluorescence microscopy, and, when excited, displayed multi color emission easily quantified and visualized. These nanoscale hybrids, coined hybridplexes, can condense pDNA and transfecting cells successfully, particularly the G5 CDs@PAMAM nanohybrids. In summary, CDs prepared in mild and smooth lab conditions, showing good optical properties, were used to prepare elegantly CDs@PAMAM nanohybrids with promising biomedical applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMartins, I., Tomás, H., Lahoz, F., & Rodrigues, J. (2021). Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery. Biomacromolecules, 22(6), 2436-2450. DOI: 10.1021/acs.biomac.1c00232pt_PT
dc.identifier.doi10.1021/acs.biomac.1c00232pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4084
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.subjectCarbon dotspt_PT
dc.subjectG4-G6pt_PT
dc.subjectPAMAM dendrimerpt_PT
dc.subjectPAMAM nanohybridspt_PT
dc.subjectEngineered fluorescentpt_PT
dc.subjectBioimagingpt_PT
dc.subjectGene deliverypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleEngineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene deliverypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.citation.endPage2450pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage2436pt_PT
oaire.citation.titleBiomacromoleculespt_PT
oaire.citation.volume22pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameViveiros Martins
person.familyNameTomás
person.familyNameLahoz
person.familyNameRodrigues
person.givenNameIvo José
person.givenNameHelena
person.givenNameFernando
person.givenNameJoão
person.identifierE-5505-2018
person.identifier556975
person.identifier.ciencia-id711C-4241-D887
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.orcid0000-0003-3818-248X
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0001-8008-1696
person.identifier.orcid0000-0003-4552-1953
person.identifier.ridE-5991-2010
person.identifier.ridS-1251-2017
person.identifier.ridB-6816-2008
person.identifier.scopus-author-id6508104177
person.identifier.scopus-author-id55161203400
person.identifier.scopus-author-id9233278800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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