Receptor-mediated gene delivery using PAMAM dendrimers conjugated with peptides recognized by mesenchymal stem cells

Santos, José L.; Pandita, Deepti; Rodrigues, João; Pêgo, Ana P.; Granja, Pedro L.; Balian, Gary; Tomás, Helena

http://hdl.handle.net/10400.13/2399

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Resumo(s)

As mesenchymalstemcells(MSCs)candifferentiateintomultiplecelltypes,thedeliveryof exogenous genes to this type of cell can be an important tool in tissue regeneration and engineering. HowevertransfectionofMSCsusingnonviralgenedeliveryvectorsisdifﬁcult,thedevelopmentofmore efﬁcientandsafeDNAvehiclesbeingnecessary.Moreover,speciﬁctransfectionofMSCsmayberequired to avoid unwanted side effects in other tissues. In this study, a novel family of gene delivery vectors based on poly(amidoamine) (PAMAM) dendrimers functionalized with peptides displaying high afﬁnity toward MSCs was prepared. The vectors were characterized with respect to their ability to neutralize, bindandcompactplasmidDNA(pDNA).ThecomplexesformedbetweenthevectorsandpDNAwere analyzedconcerningtheirsize, -potential,capacityofbeinginternalizedbycellsandtransfectionefﬁciency. Thesenewvectorsexhibitedlowcytotoxicity,receptor-mediatedgenedeliveryintoMSCsandtransfection efﬁciencies superior to those presented by native dendrimers and by partially degraded dendrimers.

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PAMAM dendrimers DNA Gene delivery Cell recognition Mesenchymal stem cells . Faculdade de Ciências Exatas e da Engenharia

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http://hdl.handle.net/10400.13/2399

Citação

Santos, J. L., Pandita, D., Rodrigues, J., Pêgo, A. P., Granja, P. L., Balian, G., & Tomás, H. (2010). Receptor-mediated gene delivery using PAMAM dendrimers conjugated with peptides recognized by mesenchymal stem cells. Molecular pharmaceutics, 7(3), 763-774.