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Receptor-mediated gene delivery using PAMAM dendrimers conjugated with peptides recognized by mesenchymal stem cells

2010-06-07Journal article Restricted access
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dc.contributor.authorSantos, José L.
dc.contributor.authorPandita, Deepti
dc.contributor.authorRodrigues, João
dc.contributor.authorPêgo, Ana P.
dc.contributor.authorGranja, Pedro L.
dc.contributor.authorBalian, Gary
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-06-17T15:11:46Z
dc.date.available2019-06-17T15:11:46Z
dc.date.issued2010-06-07
dc.description.abstractAs mesenchymalstemcells(MSCs)candifferentiateintomultiplecelltypes,thedeliveryof exogenous genes to this type of cell can be an important tool in tissue regeneration and engineering. HowevertransfectionofMSCsusingnonviralgenedeliveryvectorsisdifficult,thedevelopmentofmore efficientandsafeDNAvehiclesbeingnecessary.Moreover,specifictransfectionofMSCsmayberequired to avoid unwanted side effects in other tissues. In this study, a novel family of gene delivery vectors based on poly(amidoamine) (PAMAM) dendrimers functionalized with peptides displaying high affinity toward MSCs was prepared. The vectors were characterized with respect to their ability to neutralize, bindandcompactplasmidDNA(pDNA).ThecomplexesformedbetweenthevectorsandpDNAwere analyzedconcerningtheirsize, -potential,capacityofbeinginternalizedbycellsandtransfectionefficiency. Thesenewvectorsexhibitedlowcytotoxicity,receptor-mediatedgenedeliveryintoMSCsandtransfection efficiencies superior to those presented by native dendrimers and by partially degraded dendrimers.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSantos, J. L., Pandita, D., Rodrigues, J., Pêgo, A. P., Granja, P. L., Balian, G., & Tomás, H. (2010). Receptor-mediated gene delivery using PAMAM dendrimers conjugated with peptides recognized by mesenchymal stem cells. Molecular pharmaceutics, 7(3), 763-774.pt_PT
dc.identifier.doi10.1021/mp9002877pt_PT
dc.identifier.issn1543-8384
dc.identifier.urihttp://hdl.handle.net/10400.13/2399
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationBONE TISSUE ENGINEERING VIA LOCAL GENE DELIVERY
dc.subjectPAMAM dendrimerspt_PT
dc.subjectDNApt_PT
dc.subjectGene deliverypt_PT
dc.subjectCell recognitionpt_PT
dc.subjectMesenchymal stem cellspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleReceptor-mediated gene delivery using PAMAM dendrimers conjugated with peptides recognized by mesenchymal stem cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBONE TISSUE ENGINEERING VIA LOCAL GENE DELIVERY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BEB%2F71161%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI-2010/SFRH%2FBD%2F19450%2F2004/PT
oaire.citation.endPage774pt_PT
oaire.citation.startPage763pt_PT
oaire.citation.titleMolecular Pharmaceuticspt_PT
oaire.citation.volume7(3)pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamPOCI-2010
person.familyNameRodrigues
person.familyNamePêgo
person.familyNameTomás
person.givenNameJoão
person.givenNameAna Paula
person.givenNameHelena
person.identifier164495
person.identifier556975
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id6114-92F0-E64C
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0001-5169-328X
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridB-6816-2008
person.identifier.ridC-6942-2012
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id56053889300
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication905a2f89-0018-47d3-850d-4b09bed65b5f
relation.isAuthorOfPublication3a747114-6ab9-454b-985b-00cbf792e273
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