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Dendrimer-assisted formation of fluorescent nanogels for drug delivery and intracellular imaging

2014Journal article Restricted access
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dc.contributor.authorGonçalves, Mara
dc.contributor.authorMaciel, Dina
dc.contributor.authorCapelo, Débora
dc.contributor.authorXiao, Shili
dc.contributor.authorSun, Wenjie
dc.contributor.authorShi, Xiangyang
dc.contributor.authorRodrigues, João
dc.contributor.authorTomás, Helena
dc.contributor.authorLi, Yulin
dc.date.accessioned2019-06-21T13:53:08Z
dc.date.available2019-06-21T13:53:08Z
dc.date.issued2014
dc.description.abstractAlthough, in general, nanogels present a good biocompatibility and are able to mimic biological tissues, their unstability and uncontrollable release properties still limit their biomedical applications. In this study, a simple approach was used to develop dual-cross-linked dendrimer/alginate nanogels (AG/G5), using CaCl2 as cross-linker and amine-terminated generation 5 dendrimer (G5) as a cocrosslinker, through an emulsion method. Via their strong electrostatic interactions with anionic AG, together with cross-linker Ca(2+), G5 dendrimers can be used to mediate the formation of more compact structural nanogels with smaller size (433 ± 17 nm) than that (873 ± 116 nm) of the Ca(2+)-cross-linked AG nanogels in the absence of G5. Under physiological (pH 7.4) and acidic (pH 5.5) conditions, the sizes of Ca(2+)-cross-linked AG nanogels gradually decrease probably because of their degradation, while dual-cross-linked AG/G5 nanogels maintain a relatively more stable structure. Furthermore, the AG/G5 nanogels effectively encapsulate the anticancer drug doxorubicin (Dox) with a loading capacity 3 times higher than that of AG nanogels. The AG/G5 nanogels were able to release Dox in a sustained way, avoiding the burst release observed for AG nanogels. In vitro studies show that the AG/G5-Dox NGs were effectively taken up by CAL-72 cells (a human osteosarcoma cell line) and maintain the anticancer cytotoxicity levels of free Dox. Interestingly, G5 labeled with a fluorescent marker can be integrated into the nanogels and be used to track the nanogels inside cells by fluorescence microscopy. These findings demonstrate that AG/G5 nanogels may serve as a general platform for therapeutic delivery and/or cell imaging.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGonçalves, M., Maciel, D., Capelo, D., Xiao, S., Sun, W., Shi, X., ... & Li, Y. (2014). Dendrimer-assisted formation of fluorescent nanogels for drug delivery and intracellular imaging. Biomacromolecules, 15(2), 492-499.pt_PT
dc.identifier.doi10.1021/bm401400rpt_PT
dc.identifier.issn1525-7797
dc.identifier.urihttp://hdl.handle.net/10400.13/2418
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.relationSelf-assembled nanoparticles based on PEG-PLA-dendrimer building blocks for dual gene/drug delivery
dc.relationNEW MATERIALS FOR BIOMEDICAL APPLICATIONS OBTAINED BY THE SELF-ASSEMBLY OF DENDRIMER-SINGLE STRANDED DNA CONJUGATES
dc.subjectAntineoplastic agentspt_PT
dc.subjectCell proliferationpt_PT
dc.subjectCell survivalpt_PT
dc.subjectDendrimerspt_PT
dc.subjectDose-response Relationshippt_PT
dc.subjectDoxorubicinpt_PT
dc.subjectDrug screening assayspt_PT
dc.subjectNIH 3T3 cellspt_PT
dc.subjectPolyethylene glycolspt_PT
dc.subjectPolyethyleneiminept_PT
dc.subjectStructure-activity relationshippt_PT
dc.subjectDrug delivery systemspt_PT
dc.subjectFluorescencept_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleDendrimer-assisted formation of fluorescent nanogels for drug delivery and intracellular imagingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardTitleSelf-assembled nanoparticles based on PEG-PLA-dendrimer building blocks for dual gene/drug delivery
oaire.awardTitleNEW MATERIALS FOR BIOMEDICAL APPLICATIONS OBTAINED BY THE SELF-ASSEMBLY OF DENDRIMER-SINGLE STRANDED DNA CONJUGATES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-NAN%2F116788%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-NAN%2F112428%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F88721%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F75420%2F2010/PT
oaire.citation.endPage499pt_PT
oaire.citation.startPage492pt_PT
oaire.citation.titleBiomacromoleculespt_PT
oaire.citation.volume15(2)pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameGonçalves
person.familyNameMaciel
person.familyNameXiao
person.familyNameShi
person.familyNameRodrigues
person.familyNameTomás
person.familyNameLi
person.givenNameMara
person.givenNameDina
person.givenNameShili
person.givenNameXiangyang
person.givenNameJoão
person.givenNameHelena
person.givenNameYulin
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person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0001-5569-1038
person.identifier.ridA-3537-2013
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person.identifier.scopus-author-id55509603000
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person.identifier.scopus-author-id35192467200
person.identifier.scopus-author-id7402953116
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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