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A novelty system for biotization of plant microshoots and collection of natural compounds
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Orientador(es)
Resumo(s)
An in vitro plant microshoot culture system composed of two phases; a liquid phase
overlaid by a floating solid phase, which is described in detail herein. This system is designed to
enable the extraction of natural compounds released/disseminated into the liquid phase during
root growth, thus facilitating their processing and biochemical characterization. The solid phase
holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its
penetration into the liquid phase, where the roots are submerged. Both phases can be independently
formulated as required for growth optimization of both organisms. Considering the closed system
and known variables described in this patent, applications of the described method include testing
with pesticides, herbicides, and other similar products.
Descrição
Palavras-chave
Biotechnology Biotization Chemical analysis Double-phase medium Phenolic compounds Liquid chromatography Diode array detector Mass spectrometry . Escola Superior de Tecnologias e Gestão
Contexto Educativo
Citação
Castro, M., Ragonezi, C., de Oliveira, P., & Zavattieri, M. (2019). A novelty system for biotization of plant microshoots and collection of natural compounds. Methods and Protocols, 2(1). http://dx.doi.org/10.3390/mps2010005
Editora
MDPI