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A novelty system for biotization of plant microshoots and collection of natural compounds

2019Journal article Open access
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dc.contributor.authorCastro, Mário Rui da Costa Basílio e
dc.contributor.authorRagonezi, Carla
dc.contributor.authorOliveira, Paulo Guilherme Leandro de
dc.contributor.authorZavattieri, Maria Amely
dc.date.accessioned2022-02-04T11:50:25Z
dc.date.available2022-02-04T11:50:25Z
dc.date.issued2019
dc.description.abstractAn in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCastro, M., Ragonezi, C., de Oliveira, P., & Zavattieri, M. (2019). A novelty system for biotization of plant microshoots and collection of natural compounds. Methods and Protocols, 2(1). http://dx.doi.org/10.3390/mps2010005pt_PT
dc.identifier.doi10.3390/mps2010005pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4042
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiotechnologypt_PT
dc.subjectBiotizationpt_PT
dc.subjectChemical analysispt_PT
dc.subjectDouble-phase mediumpt_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectLiquid chromatographypt_PT
dc.subjectDiode array detectorpt_PT
dc.subjectMass spectrometrypt_PT
dc.subject.pt_PT
dc.subjectEscola Superior de Tecnologias e Gestãopt_PT
dc.titleA novelty system for biotization of plant microshoots and collection of natural compoundspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-CFL%2F71437%2F2006/PT
oaire.citation.issue1pt_PT
oaire.citation.titleMethods and Protocolspt_PT
oaire.citation.volume2pt_PT
oaire.fundingStream5876-PPCDTI
person.familyNameCastro
person.familyNameRagonezi
person.familyNameZavattieri
person.givenNameMário Rui
person.givenNameCarla
person.givenNameMaria Amely
person.identifier.ciencia-idFC12-D3F0-EF3D
person.identifier.orcid0000-0002-8543-4574
person.identifier.orcid0000-0002-1822-5473
person.identifier.orcid0000-0001-9640-8227
person.identifier.ridL-3735-2013
person.identifier.scopus-author-id36663477800
person.identifier.scopus-author-id36460033800
person.identifier.scopus-author-id35486257300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication4a188050-e705-41e2-af9c-2a67aecad273
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relation.isProjectOfPublication6d373255-42e5-4099-9487-7e2aa0f611e8
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