Insight into the role of N,N-dimethylaminoethyl methacrylate (DMAEMA) conjugation onto poly(ethylenimine): cell viability and gene transfection studies

Nouri, Alireza; Castro, Rita; Kairys, Visvaldas; Santos, José L.; Rodrigues, João; Li, Yulin; Tomás, Helena

http://hdl.handle.net/10400.13/2410

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Abstract(s)

In the present study, the effect of N,N-dimethylaminoethyl methacrylate (DMAEMA) conjugation onto branched poly(ethylenimine) (PEI) with different grafting degree was examined for gene delivery applications. The DMAEMA-grafted-PEI conjugates were characterized and complexed with plasmid DNA (pDNA) at various concentrations, and the physicochemical properties, cell viability, and in vitro transfection efficiency of the complexes were evaluated in HEK 293T cells. Computational techniques were used to analyze the interaction energies and possible binding modes between DNA and conjugates at different grafting degrees. The cytotoxicity analysis and in vitro transfection efficiency of the conjugate/pDNA complexes exhibited a beneficial effect of DMAEMA conjugation when compared to PEI alone. The computational results revealed that the DNA/vector interaction energy decreases with increasing grafting degree, which can be associated to an enhanced release of the pDNA from the carrier once inside cells. The results indicate the significance of DMAEMA conjugation onto PEI as a promising approach for gene delivery applications.

Keywords

Buffers Cell survival DNA Genetic vectors Green fluorescent proteins HEK293 cells Humans Hydrogen-ion concentration Light Magnetic resonance spectroscopy Methacrylates Microscopy fluorescence Chemical models Plasmids Polyethyleneimine Polymers Radiotion Scattering Transfection Gene transfer techniques . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira

URI

http://hdl.handle.net/10400.13/2410

Citation

Nouri, A., Castro, R., Kairys, V., Santos, J. L., Rodrigues, J., Li, Y., & Tomás, H. (2012). Insight into the role of N, N-dimethylaminoethyl methacrylate (DMAEMA) conjugation onto poly (ethylenimine): cell viability and gene transfection studies. Journal of Materials Science: Materials in Medicine, 23(12), 2967-2980.