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Insight into the role of N,N-dimethylaminoethyl methacrylate (DMAEMA) conjugation onto poly(ethylenimine): cell viability and gene transfection studies

2012Journal article Restricted access
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dc.contributor.authorNouri, Alireza
dc.contributor.authorCastro, Rita
dc.contributor.authorKairys, Visvaldas
dc.contributor.authorSantos, José L.
dc.contributor.authorRodrigues, João
dc.contributor.authorLi, Yulin
dc.contributor.authorTomás, Helena
dc.date.accessioned2019-06-19T11:24:58Z
dc.date.available2019-06-19T11:24:58Z
dc.date.issued2012
dc.description.abstractIn the present study, the effect of N,N-dimethylaminoethyl methacrylate (DMAEMA) conjugation onto branched poly(ethylenimine) (PEI) with different grafting degree was examined for gene delivery applications. The DMAEMA-grafted-PEI conjugates were characterized and complexed with plasmid DNA (pDNA) at various concentrations, and the physicochemical properties, cell viability, and in vitro transfection efficiency of the complexes were evaluated in HEK 293T cells. Computational techniques were used to analyze the interaction energies and possible binding modes between DNA and conjugates at different grafting degrees. The cytotoxicity analysis and in vitro transfection efficiency of the conjugate/pDNA complexes exhibited a beneficial effect of DMAEMA conjugation when compared to PEI alone. The computational results revealed that the DNA/vector interaction energy decreases with increasing grafting degree, which can be associated to an enhanced release of the pDNA from the carrier once inside cells. The results indicate the significance of DMAEMA conjugation onto PEI as a promising approach for gene delivery applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNouri, A., Castro, R., Kairys, V., Santos, J. L., Rodrigues, J., Li, Y., & Tomás, H. (2012). Insight into the role of N, N-dimethylaminoethyl methacrylate (DMAEMA) conjugation onto poly (ethylenimine): cell viability and gene transfection studies. Journal of Materials Science: Materials in Medicine, 23(12), 2967-2980.pt_PT
dc.identifier.doi10.1007/s10856-012-4753-9pt_PT
dc.identifier.issn0957-4530
dc.identifier.urihttp://hdl.handle.net/10400.13/2410
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.subjectBufferspt_PT
dc.subjectCell survivalpt_PT
dc.subjectDNApt_PT
dc.subjectGenetic vectorspt_PT
dc.subjectGreen fluorescent proteinspt_PT
dc.subjectHEK293 cellspt_PT
dc.subjectHumanspt_PT
dc.subjectHydrogen-ion concentrationpt_PT
dc.subjectLightpt_PT
dc.subjectMagnetic resonance spectroscopypt_PT
dc.subjectMethacrylatespt_PT
dc.subjectMicroscopy fluorescencept_PT
dc.subjectChemical modelspt_PT
dc.subjectPlasmidspt_PT
dc.subjectPolyethyleneiminept_PT
dc.subjectPolymerspt_PT
dc.subjectRadiotion Scatteringpt_PT
dc.subjectTransfectionpt_PT
dc.subjectGene transfer techniquespt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleInsight into the role of N,N-dimethylaminoethyl methacrylate (DMAEMA) conjugation onto poly(ethylenimine): cell viability and gene transfection studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2980pt_PT
oaire.citation.startPage2967pt_PT
oaire.citation.titleJournal of Materials Science: Materials in Medicinept_PT
oaire.citation.volume23(12)pt_PT
person.familyNameNouri
person.familyNameCastro
person.familyNameKairys
person.familyNameRodrigues
person.familyNameLi
person.familyNameTomás
person.givenNameAlireza
person.givenNameRita
person.givenNameVisvaldas
person.givenNameJoão
person.givenNameYulin
person.givenNameHelena
person.identifierK-9893-2013
person.identifier556975
person.identifier.ciencia-id8314-881E-4986
person.identifier.ciencia-idA716-F916-AFB2
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0003-3532-1309
person.identifier.orcid0000-0002-9337-062X
person.identifier.orcid0000-0002-5427-0175
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0001-5569-1038
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridA-2497-2013
person.identifier.ridB-6816-2008
person.identifier.ridA-4082-2013
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id6602925161
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id55718977200
person.identifier.scopus-author-id6508104177
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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