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Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography

2025-03-08Journal article Open access
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datacite.subject.fosCiências Agrárias::Ciência Animal e dos Laticínios
datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Ambiental
dc.contributor.authorAntos, Joanna
dc.contributor.authorGarcía-Cansino, Laura
dc.contributor.authorÁngeles García, María
dc.contributor.authorGinter-Kramarczyk, Dobrochna
dc.contributor.authorMarina, María Luisa
dc.contributor.authorZembrzuska, Joanna
dc.contributor.authorCâmara, José S.
dc.contributor.authorPereira, Jorge A. M.
dc.contributor.authorCâmara, José
dc.contributor.authorAugusto Machado Pereira, Jorge
dc.date.accessioned2025-07-17T09:01:47Z
dc.date.available2025-07-17T09:01:47Z
dc.date.issued2025-03-08
dc.description.abstractA μSPEed microextraction combined with ultra-performance liquid chromatography (UHPLC) with UV detection was developed for analysing six veterinary antibiotics (tetracycline, chlortetracycline, oxytetracycline, doxycycline, sulfamethoxazole, and trimethoprim) in environmental samples. To optimise extraction, 12 sorbent cartridges, sample loading cycles, volumes, and pH were assayed. The PS/DVB-RP cartridge, three 250 μL sample loading cycles, and two 50-µL elutions with acidified methanol yielded maximum efficiency. The method was validated with optimised fast chromatographic separation, showing good linearity (R2 > 0.99), precision (RSD < 20%), and recoveries between 46-86%. Detection and quantification limits ranged from 0.30-1.23 μg L−1 and 0.92-3.73 μg L−1 , respectively. The optimised μSPEed/UPLC-PDA efficiently analysed environmental water samples, requiring only 6 min extraction, 6 min analysis, and 500 μL sample, surpassing alternative methods in speed, workloads and reproducibility. The cost-effective, commercially available equipment facilitates accessibility for laboratories and adaptability for analysing selected antibiotics in diverse matrices, including food and environmental samples.eng
dc.identifier.citationAntos, J., García-Cansino, L., García, M.Á. et al. Improved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatography. Commun Chem 8, 68 (2025). https://doi.org/10.1038/s42004-025-01454-w
dc.identifier.doi10.1038/s42004-025-01454-w
dc.identifier.issn2399-3669
dc.identifier.urihttp://hdl.handle.net/10400.13/7323
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.relation.ispartofCommunications Chemistry
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectVeterinary antibiotics
dc.subjectMethodology to survey veterinary antibiotics
dc.subjectµSPEed microextraction
dc.subject.
dc.subjectCentro de Química da Madeira
dc.subjectFaculdade de Ciências Exatas e da Engenharia
dc.titleImproved methodology to survey veterinary antibiotics in environmental samples using µSPEed microextraction followed by ultraperformance liquid chromatographyeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.citation.issue1
oaire.citation.titleCommunications Chemistry
oaire.citation.volume8
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCâmara
person.familyNameAugusto Machado Pereira
person.givenNameJosé
person.givenNameJorge
person.identifierG-3003-2013
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.ciencia-idEC12-EE8F-50E8
person.identifier.orcid0000-0003-1965-3151
person.identifier.orcid0000-0003-0316-5348
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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relation.isAuthorOfPublication.latestForDiscoverye10d78be-e547-4d25-92b5-06a997ed78da
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