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Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study

2007Journal article Open access
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dc.contributor.authorSpínola, Hélder
dc.contributor.authorBruges-Armas, Jácome
dc.contributor.authorBrehm, António
dc.date.accessioned2017-01-26T16:29:50Z
dc.date.available2017-01-26T16:29:50Z
dc.date.issued2007
dc.description.abstractSix hundred twenty-one samples from Portugal, the Cabo Verde archipelago, and Guinea-Bissau were typed for HLA-A, HLA-B, and HLADRB1usingthepolymerasechainreaction–sequence-specificoligonucleotide probe (PCR-SSOP) method and the sequence-based typing (SBT) method to characterizeandcomparediscrepanciesbetweenthetwomethods.Fifty-three alleles (4.27% of 1,242 chromosomes typed) identified by the PCR-SSOP method were not concordant with the results obtained using the SBT method. Thirty-four (2.74% of total chromosomes typed) PCR-SSOP mistyping results were discrepancies inside the same allele group and 19 others (1.53% of total chromosomes typed) were relative to nonconcordant results between different groups. PCR-SSOP allele mistyping is the result of interpretation difficulties resulting from less intense, absent, or dubious hybridization patterns. Noncommercial PCR-SSOP procedures are highly exigent on the technicians’ experience and the availability of properly calibrated high-precision equipment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSpínola, H., Bruges-Armas, J., & Brehm, A. (2007). Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: A Case Study. Human Biology, 79 (5), 537-543.pt_PT
dc.identifier.issn0018-7143
dc.identifier.urihttp://hdl.handle.net/10400.13/1351
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWayne State University Presspt_PT
dc.subjectHLApt_PT
dc.subjectPolymerase chain reaction - sequence-specific - oligonucleotide probe (PCR-SSOP) techniquept_PT
dc.subjectSequence-based typing (SBT) techniquept_PT
dc.subjectPortugalpt_PT
dc.subjectCabo Verdept_PT
dc.subjectGuiné-Bissaupt_PT
dc.subjectAzores islands (Portugal)pt_PT
dc.subject.
dc.titleDiscrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FBIA-BCM%2F60440%2F2004/PT
oaire.citation.conferencePlaceEstados Unidos da América, Michiganpt_PT
oaire.citation.endPage543pt_PT
oaire.citation.startPage537pt_PT
oaire.citation.titleHuman Biologypt_PT
oaire.citation.volume79 (5)pt_PT
oaire.fundingStreamPOCI
person.familyNameSpínola
person.familyNameArmas
person.familyNameBrehm
person.givenNameHélder
person.givenNameJacome
person.givenNameAntonio
person.identifier682991
person.identifier.ciencia-id8413-FBC3-5584
person.identifier.ciencia-id3613-8D1F-0EDF
person.identifier.orcid0000-0002-6455-1595
person.identifier.orcid0000-0001-7671-6521
person.identifier.orcid0000-0003-3168-5340
person.identifier.scopus-author-id10242534600
person.identifier.scopus-author-id55931428200
person.identifier.scopus-author-idAuthor ID 7006085833
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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